Microbiomes of the normal middle ear and ears with chronic otitis media

Laryngoscope - Tập 127 Số 10 - 2017
Shujiro Minami1, Hideki Mutai1, Tomoko Suzuki1, Arata Horii2, Naoki Oishi3, Koichiro Wasano4, Motoyasu Katsura5, Fujinobu Tanaka6, Tetsuya Takiguchi7, Masato Fujii1, Kimitaka Kaga8,1
1National Hospital Organization Tokyo Medical Center, National Institute of Sensory Organs, Tokyo, Japan
2Niigata University, Department of Otolaryngology Head and Neck Surgery, Nigata, Japan
3Keio University, School of Medicine, Department of Otolaryngology, Keio, Japan
4Japanese Red Cross Shizuoka Hospital, Department of Otolaryngology, Shizuoka, Japan
5National Hospital Organization Ureshino Medical Center, Department of Otolaryngology, Ureshino, Japan
6National Hospital Organization Nagasaki Medical Center, Department of Otolaryngology, Nagasaki, Japan
7National Hospital Organization Kanazawa Medical Center, Department of Otolaryngology, Kanazawa, Japan
8International University of Health and Welfare, Center for Speech and Hearing Disorders, Tochigi, Japan

Tóm tắt

ObjectiveThe aim of this study was to profile and compare the middle ear microbiomes of human subjects with and without chronic otitis media.Study DesignProspective multicenter cohort study.MethodsAll consecutive patients undergoing tympanoplasty surgery for chronic otitis media or ear surgery for conditions other than otitis media were recruited. Sterile swab samples were collected from the middle ear mucosa during surgery. The variable region 4 of the 16S rRNA gene in each sample were amplified using region‐specific primers adapted for the Illumina MiSeq sequencer (Illumina, CA, USA)). The sequences were subjected to local blast and classified using Metagenome@KIN (World Fusion, Tokyo, Japan).ResultsIn total, 155 participants were recruited from seven medical centers. Of these, 88 and 67 had chronic otitis media and normal middle ears, respectively. The most abundant bacterial phyla on the mucosal surfaces of the normal middle ears were Proteobacteria, followed by Actinobacteria, Firmicutes, and Bacteroidetes. The children and adults with normal middle ears differed significantly in terms of middle ear microbiomes. Subjects with chronic otitis media without active inflammation (dry ear) had similar middle ear microbiomes as the normal middle ears group. Subjects with chronic otitis media with active inflammation (wet ear) had a lower prevalence of Proteobacteria and a higher prevalence of Firmicutes than the normal middle ears.ConclusionThe human middle ear is inhabited by more diverse microbial communities than was previously thought. Alteration of the middle ear microbiome may contribute to the pathogenesis of chronic otitis media with active inflammation.Level of Evidence2b. Laryngoscope, 127:E371–E377, 2017

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Laryngoscope

Tập 127 Số 10

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