Microbial diversity in chronic open wounds

Wound Repair and Regeneration - Tập 17 Số 2 - Trang 163-172 - 2009
Daniel N. Frank1,2, Annette B. Wysocki3,4, Dee Dee Specht‐Glick5, Alejandro P. Rooney6, Robert A. Feldman7, Allison L. St. Amand1, Norman R. Pace1, Jonathan D. Trent8
1Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado
2Mucosal and Vaccine Research Program Colorado,
3Division of Plastic Surgery, Department of Surgery, University of Mississippi Medical Center, Jackson, MS,
4School of Nursing, and
5St. Patrick Hospital Wound Care Center, Missoula, Montana,
6USDA Microbial Genomics and Bioprocessing Research Unit, Peoria, Illinois,
7SymBio Corporation, Menlo Park, California, and
8Bioengineering Branch, NASA Ames Research Center, Moffett Field, California

Tóm tắt

ABSTRACT

Chronic wounds expose the dermal matrix and underlying tissue to a diversity of microbes from the body and surrounding environment. We determined the microbial diversity of 19 chronic wounds using both molecular methods (sequence analysis of rRNA genes) and routine clinical culturing methods using swab samples. We identified 93 phylotypes in 2,653 rRNA clone sequences and found that compared with other environments, the microbial diversity of chronic wounds is relatively well characterized, i.e., 95% of sequences have ≥97% identity with known human commensals. In total, 75% of sequences belonged to four well‐known wound‐associated phylotypes: Staphylococcus (25%), Corynebacterium (20%), Clostridiales (18%), and Pseudomonas (12%). Approximately 0.5% of sequences (seven phylotypes) belonged to potentially new species. Individual wound samples contained four to 22 phylotypes, but in all wounds only a few (one to three) phylotypes were dominant. In more than half the wound specimens, polymerase chain reaction and culturing methods gave different diversity and dominance information about the microbes present. This exploratory study suggests that combining molecular and culturing methods provides a more complete characterization of the microbial diversity of chronic wounds, and can thereby expand our understanding of how microbiology impacts chronic wound pathology and healing.

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Wound Repair and Regeneration

Tập 17 Số 2

163-172

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