MicroRNA markers for forensic body fluid identification obtained from microarray screening and quantitative RT-PCR confirmation

Dmitry Zubakov1, Anton W. M. Boersma2, Ying Choi1, Patricia F. van Kuijk2, Erik A.C. Wiemer2, Manfred Kayser1
1Department of Forensic Molecular Biology, Erasmus University Medical Center Rotterdam, Rotterdam, The Netherlands
2Department of Medical Oncology, Josephine Nefkens Institute, Erasmus University Medical Center Rotterdam, Rotterdam, The Netherlands

Tóm tắt

Từ khóa


Tài liệu tham khảo

Bartel DP (2004) MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116:281–297

Rosenfeld N, Aharonov R, Meiri E, Rosenwald S, Spector Y, Zepeniuk M, Benjamin H, Shabes N, Tabak S, Levy A, Lebanony D, Goren Y et al (2008) MicroRNAs accurately identify cancer tissue origin. Nat Biotechnol 26:462–469

Sood P, Krek A, Zavolan M, Macino G, Rajewsky N (2006) Cell-type-specific signatures of microRNAs on target mRNA expression. Proc Natl Acad Sci U S A 103:2746–2751

Liang Y, Ridzon D, Wong L, Chen C (2007) Characterization of microRNA expression profiles in normal human tissues. BMC Genomics 8:166

Kent EJ, Elliot DA, Miskelly GM (2003) Inhibition of bleach-induced luminol chemiluminescence. J Forensic Sci 48(1):64–67

Keating SM, Higgs DF (1994) The detection of amylase on swabs from sexual assault cases. J Forensic Sci Soc 34(2):89–93

Juusola J, Ballantyne J (2007) mRNA profiling for body fluid identification by multiplex quantitative RT-PCR. J Forensic Sci 52:1252–1262

Zubakov D, Hanekamp E, Kokshoorn M, van Ijcken W, Kayser M (2008) Stable RNA markers for identification of blood and saliva stains revealed from whole genome expression analysis of time-wise degraded samples. Int J Legal Med 122:135–142

Zubakov D, Kokshoorn M, Kloosterman A, Kayser M (2009) New markers for old stains: stable mRNA markers for blood and saliva identification from up to 16-year-old stains. Int J Legal Med 123:71–74

Pothof J, Verkaik NS, van IW W, EA Ta VT, van der Horst GT, Jaspers NG, van Gent DC, Hoeijmakers JH, Persengiev SP (2009) MicroRNA-mediated gene silencing modulates the UV-induced DNA-damage response. Embo J 28:2090–2099

Saeed AI, Sharov V, White J, Li J, Liang W, Bhagabati N, Braisted J, Klapa M, Currier T, Thiagarajan M, Sturn A, Snuffin M et al (2003) TM4: a free, open-source system for microarray data management and analysis. Biotechniques 34:374–378

Tibshirani R, Hastie T, Narasimhan B, Chu G (2002) Diagnosis of multiple cancer types by shrunken centroids of gene expression. Proc Natl Acad Sci U S A 99:6567–6572

Hanson EK, Lubenow H, Ballantyne J (2009) Identification of forensically relevant body fluids using a panel of differentially expressed microRNAs. Anal Biochem 387:303–314

Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND et al (2007) A mammalian microRNA expression atlas based on small RNA library sequencing. Cell 129:1401–1414