MicroRNA Gene Expression Deregulation in Human Breast Cancer

Cancer Research - Tập 65 Số 16 - Trang 7065-7070 - 2005
Marilena V. Iorio1, Manuela Ferracin2,3, Chang‐Gong Liu4,5, Angelo Veronese2,3, Riccardo Spizzo2,3, Silvia Sabbioni2,3, Eros Magri2,3, Massimo Pedriali2,3, Muller Fabbri4, Manuela Campiglio6, Sylvie Mènard6, Juan Palazzo7,8, Anne Rosenberg9, Piero Musiani10, Stefano Volinia4, Italo Nenci2,3, George A. Calin4,5, Patrizia Querzoli2,3, Massimo Negrini2,3, Carlo M. Croce4
1Comprehensive Cancer Center, Ohio State University, Columbus, Ohio 43210, USA.
22Dipartimento di Medicina Sperimentale e Diagnostica, e Centro Interdipartimentale per la Ricerca sul Cancro, Università di Ferrara, Ferrara, Italy;
3University of Ferrara.
41Comprehensive Cancer Center, Ohio State University, Columbus, Ohio;
5Experimental Therapeutics
63Molecular Targeting Unit, Department of Experimental Oncology, Istituto Nazionale Tumori, Milan, Italy; Departments of
74Pathology, Anatomy and Cell Biology and
8Thomas Jefferson University
95Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania; and
106Ce.S.I. Aging Research Center, Chieti, Italy

Tóm tắt

Abstract MicroRNAs (miRNAs) are a class of small noncoding RNAs that control gene expression by targeting mRNAs and triggering either translation repression or RNA degradation. Their aberrant expression may be involved in human diseases, including cancer. Indeed, miRNA aberrant expression has been previously found in human chronic lymphocytic leukemias, where miRNA signatures were associated with specific clinicobiological features. Here, we show that, compared with normal breast tissue, miRNAs are also aberrantly expressed in human breast cancer. The overall miRNA expression could clearly separate normal versus cancer tissues, with the most significantly deregulated miRNAs being mir-125b, mir-145, mir-21, and mir-155. Results were confirmed by microarray and Northern blot analyses. We could identify miRNAs whose expression was correlated with specific breast cancer biopathologic features, such as estrogen and progesterone receptor expression, tumor stage, vascular invasion, or proliferation index.

Từ khóa


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Cancer Research

Tập 65 Số 16

7065-7070

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