Micro-Extraction of Commonly Abused Benzodiazepines for Urinary Screening by Liquid Chromatography

Annals of Clinical Biochemistry - Tập 34 Số 1 - Trang 61-67 - 1997
Kim Wolff1, Deborah J Garretty1, Alastair Hay1
1School of Medicine, Division of Clinical Sciences, University of Leeds, Old Medical School, Leeds LS2 9JT, UK

Tóm tắt

We have developed a micro-extraction procedure for the analysis of seven commonly prescribed benzodiazepines (chlordiazepoxide, diazepam, lorazepam, nitrazepam, nordiazepam, oxazepam, and temazepam) in urine using liquid chromatography. The method is reliable and sensitive, uses small volumes (100μL) of urine and is suitable for the detection and quantification of low concentrations of benzodiazepines. The micro-extraction procedure allowed rapid sample processing, which is important for routine sample handling. The limit of detection for the seven benzodiazepines ranged from 0·10–0·71 mg/L and recovery of the different benzodiazepines was good, ranging from 70–105%. Between-and within-assay coefficients of variation ranged from 6·3% to 13·8%, and 2% to 3·5%, respectively. Chlordiazepoxide chromatographed poorly (between assay coefficient of variation 35·4%, within-assay 7%), and we set the cut-off value for this compound at 5·0 mg/L.

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Annals of Clinical Biochemistry

Tập 34 Số 1

61-67

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