Mice exposed in situ to urban air pollution exhibit pulmonary alterations in gene expression in the lipid droplet synthesis pathways

Environmental and Molecular Mutagenesis - Tập 54 Số 4 - Trang 240-249 - 2013
Andrea Rowan‐Carroll1, Sabina Halappanavar1, Andrew Williams1, Christophers M. Somers2, Carole L. Yauk1
1Environmental Health Science and Research Bureau, Health Canada, Ottawa, Ontario, Canada
2Department of Biology; University of Regina; Saskatchewan; Canada

Tóm tắt

It is clear that particulate air pollution poses a serious risk to human health; however, the underlying mechanisms are not completely understood. We investigated pulmonary transcriptional responses in mice following in‐situ exposure to ambient air in a heavily industrialized urban environment. Mature C57BL/CBA male mice were caged in sheds near two working steel mills and a major highway in Hamilton, Ontario, Canada in the spring/summer of 2004. Control mice were housed in the same environment, but received only high‐efficiency particle filtered air (HEPA). Whole lung tissues were collected from mice exposed for 3, 10, or for 10 weeks followed by 6 weeks recovery in the laboratory (16 weeks). DNA microarrays were used to profile changes in pulmonary gene expression. Transcriptional profiling revealed changes in the expression of genes implicated in the lipid droplet synthesis (Plin I, Dgat2, Lpl, S3‐12,andAgpat2), and antioxidant defense (Ucp1) pathways in mice breathing unfiltered air. We postulate that exposure to urban air, containing an abundance of particulate matter adsorbed with polycyclic aromatic hydrocarbons, triggers lipid droplet (holding depots for lipids and malformed/excess proteins tagged for degradation) synthesis in the lungs, which may act to sequester particulates. Increased lipid droplet synthesis could lead to endogenous/stressor‐induced production of reactive oxygen species and activation of antioxidant mechanisms. Further investigation into the stimulation of lipid droplet synthesis in the lung in response to air pollution and the resulting health implications is warranted. Environ. Mol. Mutagen. 54:240–249, 2013. © 2013 Wiley Periodicals, Inc.

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Environmental and Molecular Mutagenesis

Tập 54 Số 4

240-249

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