Methods for inoculating field crops with mycorrhizal fungi

Annals of Applied Biology - Tập 99 Số 3 - Trang 247-253 - 1981
D. S. Hayman1, E. Jane Morris1, Rae Page1
1Soil Microbiology Department, Rothamsted Experimental Station, Harpenden, Herts., AL5 2JQ

Tóm tắt

SUMMARYFour methods were compared for inoculating red clover with selected mycorrhizal fungi when sown in a field containing an indigenous mycorrhizal population. The largest amount of mycorrhizal infection (around 65% of root length infected) was obtained by placing inoculum with the seeds in furrows. The inoculum used was standard soil inoculum from stock plant cultures spread by hand or the same inoculum concentrated to about one seventh by wet‐sieving, and then fluid‐drilled. The effectiveness of multiseeded pellets (seeds stuck onto pellets of soil inoculum) applied broadcast was more variable, infection ranging widely around an average of 30%. Applying both soil inoculum and seeds broadcast produced just under 10% infection, similar to that in the controls given autoclaved inoculum. Seedling establishment, in contrast, was‐better where seeds were applied broadcast than in furrows. It seemed therefore that multiseeded pellets might be the best compromise for achieving reasonable infection in most plants, but fluid drilling had the advantages of greatly reducing the amount of inoculum needed and of readily combining seeds and inoculum in a single carrier.

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Tài liệu tham khảo

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Annals of Applied Biology

Tập 99 Số 3

247-253

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