Methods for inoculating field crops with mycorrhizal fungi
Tóm tắt
Four methods were compared for inoculating red clover with selected mycorrhizal fungi when sown in a field containing an indigenous mycorrhizal population. The largest amount of mycorrhizal infection (around 65% of root length infected) was obtained by placing inoculum with the seeds in furrows. The inoculum used was standard soil inoculum from stock plant cultures spread by hand or the same inoculum concentrated to about one seventh by wet‐sieving, and then fluid‐drilled. The effectiveness of multiseeded pellets (seeds stuck onto pellets of soil inoculum) applied broadcast was more variable, infection ranging widely around an average of 30%. Applying both soil inoculum and seeds broadcast produced just under 10% infection, similar to that in the controls given autoclaved inoculum. Seedling establishment, in contrast, was‐better where seeds were applied broadcast than in furrows. It seemed therefore that multiseeded pellets might be the best compromise for achieving reasonable infection in most plants, but fluid drilling had the advantages of greatly reducing the amount of inoculum needed and of readily combining seeds and inoculum in a single carrier.
Từ khóa
Tài liệu tham khảo
ELMES R.&MOSSE B.(1980).Vesicular‐arbuscular mycorrhiza: nutrient film technique.Rothamsted Experimental Station Report for 1979 Part 1p.188.
HAYMAN D. S.&MOSSE B.(1977).Mycorrhizal studies: inoculation methods.Rothamsted Experimental Station Report for 1976 Part 1p.286.
MIKOLA P., 1980, Tropical Mycorrhiza Research, 288
MOSSE H., 1980, Tropical Mycorrhiza Research, 213
OLSEN S. T. COLE C. U. WATANABE F. S.&DEAN L. A.(1954).Estimation of available phosphorus in soils by extraction with sodium bicarbonate.Circular 939 United States Department of Agriculture.
POWELL C. L., 1977, Proceedings of the Ruakura Farmers' 29th Conference, Hamilton, New Zealand, 24
SALTER P. J., 1978, Advances in Agriculture, 16
SANDERS F. E., 1975, Endomycorrhizas, 626
WITTY J. F.&HAYMAN D. S.(1978).Slurry inoculation of VA mycorrhiza.Rothamsted Experimental Station Report for 1977 Part 1 pp.239–240.