Method for improving the quality of genomic DNA obtained from minute quantities of tissue and blood samples using Chelex 100 resin

Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: a laboratory manual. Cold Spring Harbor: Cold Spring Harbor Laboratory; 1989.

Ivanova NV, Dewaard JR, Hebert PDN. An inexpensive, automation-friendly protocol for recovering high-quality DNA. Mol Ecol Notes. 2006;6(4):998–1002.

Hawkins T. DNA purification and isolation using magnetic particles. Google Patents; 1998.

Uhlen M. Magnetic separation of DNA. Nature. 1989;340(6236):733–4.

Berensmeier S. Magnetic particles for the separation and purification of nucleic acids. Appl Microbiol Biotechnol. 2006;73(3):495–504.

Singer-Sam J, Tanguay RL, Rijggs AO. Use of Chelex to improve PC signal from a small number of cells, Amplifications: A Forum for PCR Users; 1989. p. 11.

Walsh PS, Metzger DA, Higuchi R. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. BioTechniques. 1991;10(4):506–13.

Griffiths R, Double MC, Orr K, Dawson RJG. A DNA test to sex most birds. Mol Ecol. 1998;7(8):1071–5.

Soderstrom K, Qin W, Leggett MH. A Minimally-Invasive Procedure for Sexing Young Zebra Finches. J Neurosci Methods. 2007;164(1):116–9.

Al-Soud WA, Rådström P. Purification and Characterization of PCR-Inhibitory Components in Blood Cells. J Clin Microbiol. 2001;39(2):485–93.

Crouse J, Amorese D. Ethanol Precipitation: Ammonium Acetate as an Alternative to Sodium Acetate. Focus. 1987;9(2):2.

Pitcher DG, Saunders NA, Owen RJ. Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol. 1989;8(4):151–6.

Giraffa G, Rossetti L, Neviani E. An evaluation of chelex-based DNA purification protocols for the typing of lactic acid bacteria. J Microbiol Methods. 2000;42(2):175–84.

Hailemariam Z, Ahmed JS, Clausen P-H, Nijhof AM. A comparison of DNA extraction protocols from blood spotted on FTA cards for the detection of tick-borne pathogens by Reverse Line Blot hybridization. Ticks Tick-borne Dis. 2017;8(1):185–9.

Lin JT, Ubalee R, Lon C, Balasubramanian S, Kuntawunginn W, Rahman R, et al. Microscopic Plasmodium falciparum Gametocytemia and Infectivity to Mosquitoes in Cambodia. J Infect Dis. 2016;213(9):1491–4.

Liu L, Wang CL, Peng WY, Yang J, Lan MQ, Zhang B, et al. Direct DNA extraction method of an obligate parasitic fungus from infected plant tissue. Gen Mol Res. 2015;14(4):18546–51.

Mseddi F, Jarboui MA, Sellami A, Sellami H, Ayadi A. A rapid and easy method for the DNA extraction from Cryptococcus neoformans. Biol Proc Online. 2011;13:5.

Musapa M, Kumwenda T, Mkulama M, Chishimba S, Norris DE, Thuma PE, et al. A Simple Chelex Protocol for DNA Extraction from Anopheles spp. J Vis Exp. 2013;(71):e3281.

Mygind T, Birkelund S, Birkebæk NH, Østergaard L, Jensen JS, Christiansen G. Determination of PCR efficiency in chelex-100 purified clinical samples and comparison of real-time quantitative PCR and conventional PCR for detection of Chlamydia pneumoniae. BMC Microbiol. 2002;2(1):17.

Ellegren H. Linkage mapping of the apolipoprotein A-I (APOA1) gene to pig Chromosome 9. Mamm Genome. 1994;5(1):58–9.

Sweet D, Lorente M, Valenzuela A, Lorente J, Alvarez JC. Increasing DNA extraction yield from saliva stains with a modified Chelex method. Forensic Sci Int. 1996;83(3):167–77.

de Lamballerie X, Zandotti C, Vignoli C, Bollet C, de Micco P. A one-step microbial DNA extraction method using "Chelex 100" suitable for gene amplification. Res Microbiol. 1992;143(8):785–90.

Polski JM, Kimzey S, Percival RW, Grosso LE. Rapid and effective processing of blood specimens for diagnostic PCR using filter paper and Chelex-100. Mol Pathol. 1998;51(4):215–7.

Sepp R, Szabo I, Uda H, Sakamoto H. Rapid techniques for DNA extraction from routinely processed archival tissue for use in PCR. J Clin Pathol. 1994;47(4):318–23.

Yue GH, Orban L. A simple and affordable method for high-throughput DNA extraction from animal tissues for polymerase chain reaction. Electrophoresis. 2005;26(16):3081–3.