Methanogen communities and <i>Bacteria</i> along an ecohydrological gradient in a northern raised bog complex

Wiley - Tập 7 Số 10 - Trang 1547-1557 - 2005
Heli Juottonen1, Pierre E. Galand1,2, Eeva‐Stiina Tuittila3, Jukka Laine4,3, Hannu Fritze5, Kim Yrjälä1
1Department of Biological and Environmental Sciences, General Microbiology, PO Box 56, 00014 University of Helsinki, Finland.
2Département de Biologie, Université Laval, Québec,Québec, G1K 7P4, Canada
3Peatland Ecology Group, Department of Forest Ecology, PO Box 27, 00014 University of Helsinki, Helsinki, Finland.
4Finnish Forest Research Institute, Parkano Research Station, Kaironiementie 54, 39700 Parkano, Finland
5Finnish Forest Research Institute, Vantaa Research Centre, PO Box 18, 01301 Vantaa, Finland,

Tóm tắt

SummaryMires forming an ecohydrological gradient from nutrient‐rich, groundwater‐fed mesotrophic and oligotrophic fens to a nutrient‐poor ombrotrophic bog were studied by comparing potential methane (CH4) production and methanogenic microbial communities. Methane production was measured from different depths of anoxic peat and methanogen communities were detected by detailed restriction fragment length polymorphism (RFLP) analysis of clone libraries, sequencing and phylogenetic analysis. Potential CH4 production changed along the ecohydrological gradient with the fens displaying much higher production than the ombrotrophic bog. Methanogen diversity also decreased along the gradient. The two fens had very similar diversity of methanogenic methyl‐coenzyme M reductase gene (mcrA), but in the upper layer of the bog the methanogen diversity was strikingly lower, and only one type of mcrA sequence was retrieved. It was related to the Fen cluster, a group of novel methanogenic sequences found earlier in Finnish mires. Bacterial 16S rDNA sequences from the fens fell into at least nine phyla, but only four phyla were retrieved from the bog. The most common bacterial groups were Deltaproteobacteria, Verrucomicrobia and Acidobacteria.

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Tài liệu tham khảo

10.1890/0012-9658(1999)080[2170:PMCONC]2.0.CO;2

Ahti T., 1968, Vegetation zones and their sections in northwestern Europe, Ann Bot Fenn, 5, 169

10.2307/3242073

10.1128/AEM.65.4.1731-1737.1999

Bartlet K.B., 1993, Review and assessment of methane emissions from wetlands, Chemosphere, 26, 261, 10.1016/0045-6535(93)90427-7

10.1080/713851165

10.2307/3545942

10.1046/j.1462-2920.2002.00337.x

10.1111/j.1574-6941.2001.tb00793.x

10.1128/AEM.70.11.6559-6568.2004

10.1029/1999GB001221

10.1128/AEM.65.11.5042-5049.1999

Clymo R.S., 1984, The limits to peat bog growth, Phil Trans R Soc Lond B, 303, 605, 10.1098/rstb.1984.0002

Duddleston K.N., 2002, Anaerobic microbial biogeochemistry in a northern bog; acetate as a dominant metabolic end product, Global Biogeochem Cycles, 16, 1063, 10.1029/2001GB001402

10.1128/AEM.64.3.871-879.1998

10.1007/s002489900113

10.1016/S0038-0717(03)00053-1

10.1007/s00248-003-1016-9

10.1111/j.1574-6941.2002.tb01033.x

10.1046/j.1462-2920.2003.00520.x

Galand P.E. Juottonen H. Fritze H. andYrjälä K.(2005a)Methanogen communities in a drained bog: effect of ash fertilization.Microb Ecol(in press).

Galand P.E. Fritze H. Conrad R. andYrjälä K.(2005b)Pathways of methanogenesis and diversity of methanogenic Archaea in three boreal peatland ecosystems.Appl Environ Microbiol71:2195–2198.

10.2307/1941811

10.1128/AEM.62.2.668-675.1996

10.1016/S0723-2020(85)80031-X

10.1128/AEM.69.1.74-83.2003

10.1016/S0016-7037(99)00321-X

10.1046/j.1365-2745.2002.00677.x

10.1111/j.1574-6941.2000.tb00753.x

10.1080/11956860.1996.11682334

10.1111/j.1462-2920.2004.00634.x

Krebs C.J., 1998, Ecological Methodology

Laine J., 1996, Peatlands in Finland, 10

Laine J., 1996, Effect of water level drawdown in northern peatlands on the global climatic warming, Ambio, 25, 179

Laine J., 2004, Lakkasuo – a guide to mire ecosystem, University of Helsinki Department of Forest Ecology Publications, 31, 1

Liedenpohja M., 1981, Avosuotyyppien kasvillisuus, kasvibiomassa ja tuotos Janakkalan Suurisuolla [Summary: vegetation, biomass and production of fens in Suurisuo mire, Janakkala, Southern Finland], Suo, 32, 100

10.1046/j.1462-2920.2001.00179.x

10.1099/00221287-148-11-3521

McDonald I.R., 1999, Molecular ecological analysis of methanogens and methanotrophs in blanket bog peat, Microb Ecol, 38, 225, 10.1007/s002489900172

10.1007/s00248-002-0008-5

10.1128/MMBR.66.4.592-616.2002

10.1128/AEM.68.3.1446-1453.2002

10.1111/j.1574-6968.1999.tb13534.x

10.1128/AEM.67.4.1874-1884.2001

10.1029/97GB02732

10.1007/BF01574689

Schink B., 2002, The Prokaryotes

10.1128/AEM.64.12.4870-4876.1998

Seppä H., 1996, Peatlands in Finland., 27

10.1016/S0168-6496(03)00165-X

10.1046/j.1365-2486.2003.00655.x

10.1099/00221287-144-9-2377

Tolonen K., 1967, Ueber die Entwicklung der Moore im Finnischen Nordkarelien, Ann Bot Fenn, 4, 219

10.1029/2000GB001312

10.1016/0169-5347(95)90007-1

Van de Peer Y., 1994, treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment, Comput Appl Biosci, 10, 569

10.1111/plb.1997.46.2.117

10.1016/0038-0717(94)00183-2

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Wiley

Tập 7 Số 10

1547-1557

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