Mesenchymal Stem Cells Inhibit the Expression of CD25 (Interleukin‐2 Receptor) and CD38 on Phytohaemagglutinin‐Activated Lymphocytes

Scandinavian Journal of Immunology - Tập 60 Số 3 - Trang 307-315 - 2004
Katarina Le Blanc1, Ida Rasmusson, Cecilia Götherström, Catharina D. Seidel, Berit Sundberg, Mikael Sundin, Ingvar Rosendahl, Charlotte Tammik, Olle Ringdén
1Division of Clinical Immunology, Huddinge University Hospital, Karolinska Institutet, Stockholm, Sweden. [email protected]

Tóm tắt

AbstractMesenchymal stem cells (MSC) are immunomodulatory and inhibit lymphocyte proliferation. We studied surface expression of lymphocyte activation markers and secreted cytokines, when lymphocytes were activated in the presence of MSC. MSC suppressed the proliferation of phytohaemagglutinin (PHA)‐stimulated CD3+, CD4+ and CD8+ lymphocytes. MSC significantly reduced the expression of activation markers CD25, CD38 and CD69 on PHA‐stimulated lymphocytes. Mixed lymphocyte culture (MLC) supernatants containing MSC suppressed proliferation of MLC and PHA‐stimulated lymphocytes dose‐dependently. MSC secrete osteoprotegerin (OPG), but not hepatocyte growth factor (HGF) or transforming growth factor‐β (TGF‐β). Stromal‐cell‐derived factor‐1 (SDF‐1) is not expressed on the cell surface. A recent report suggested that T‐cell suppression by MSC is mediated by HGF and TGF‐β. MSC suppression was not restored by the addition of neutralizing antibodies against SDF‐1, OPG, HGF or TGF‐β, alone or in combination. Addition of guanosine to PHA‐stimulated lymphocyte cultures containing MSC did not affect lymphocyte proliferation. The immunosuppressive effects of cyclosporine and MSC did not interfere, when present in the cultures of PHA‐activated lymphocytes. In summary, human MSC suppress proliferation of both CD4+ and CD8+ lymphocyte and decrease the expression of activation markers.

Từ khóa


Tài liệu tham khảo

10.1097/00007890-196803000-00009

10.1016/S0074-7696(08)60092-3

10.1016/8756-3282(92)90364-3

10.1126/science.276.5309.71

10.1126/science.284.5411.143

10.1016/8756-3282(92)90363-2

10.1002/(SICI)1097-4652(199807)176:1<57::AID-JCP7>3.0.CO;2-7

10.1016/S0301-472X(02)00820-2

10.1097/01.TP.0000045055.63901.A9

10.1016/S0301-472X(03)00110-3

10.1046/j.1365-3083.2003.01176.x

10.1016/S0301-472X(01)00769-X

10.1097/01.TP.0000082540.43730.80

10.1182/blood.V99.10.3838

10.1182/blood-2002-07-2104

Frassoni F, 2002, Expanded mesenchymal stem cells (MSC), co‐infused with HLA identical hematopoietic stem cell transplants, reduce acute and chronic graft‐versus‐host disease: a matched pair analysis, Bone Marrow Transplant, 29, S2.

10.1007/BF02724045

10.1038/75022

10.1126/science.8342023

10.1016/S0301-472X(00)00482-3

10.1146/annurev.immunol.20.100301.064753

10.1016/S0092-8674(00)81569-X

10.1074/jbc.275.19.14388

10.1097/00007890-199911150-00024

Kushida T, 2000, Treatment of intractable autoimmune diseases in MRL/ipr mice using a new strategy for allogeneic bone marrow transplantation, Blood, 95, 1862, 10.1182/blood.V95.5.1862.005k27_1862_1868

10.1182/blood.V95.11.3620

10.1097/00007890-200006270-00006

10.1097/00007890-200208150-00009

10.1016/S0140-6736(04)16104-7

10.1083/jcb.123.1.223

10.1046/j.1365-2567.2001.01186.x

10.1046/j.1523-1755.1998.00870.x

10.1136/heart.83.4.450

Beppu K, 2001, Hepatocyte growth factor production by peripheral mononuclear cells of recurrent cancer patients, Anican Res, 21, 2195

10.1016/0270-9139(94)90862-1

10.1006/bbrc.2000.4223

Thông tin
Thông tin xuất bản

Scandinavian Journal of Immunology

Tập 60 Số 3

307-315

Thông tin tác giả