Measuring ventral nerve cord stiffness in live flat-dissected Drosophila embryos by atomic force microscopy

STAR Protocols - Tập 3 - Trang 101901 - 2022
Katerina Karkali1, Ignasi Jorba2,3,4, Daniel Navajas2,3,4, Enrique Martin-Blanco1
1Instituto de Biología Molecular de Barcelona, Consejo Superior de Investigaciones Científicas, 08028 Barcelona, Spain
2Institute for Bioengineering of Catalonia, 08028 Barcelona, Spain
3CIBER de Enfermedades Respiratorias (CIBERES), 28029 Madrid, Spain
4Facultat de Medicina i Ciencies de la Salut, Universitat de Barcelona, 08036 Barcelona, Spain

Tài liệu tham khảo

Alcaraz, 2003, Microrheology of human lung epithelial cells measured by atomic force microscopy, Biophys. J., 84, 2071, 10.1016/S0006-3495(03)75014-0

Andreu, 2014, Heterogeneous micromechanical properties of the extracellular matrix in healthy and infarcted hearts, Acta Biomater., 10, 3235, 10.1016/j.actbio.2014.03.034

Crews, 2019, Drosophila embryonic CNS development: neurogenesis, gliogenesis, cell fate, and differentiation, Genetics, 213, 1111, 10.1534/genetics.119.300974

Gavara, 2016, Combined strategies for optimal detection of the contact point in AFM force-indentation curves obtained on thin samples and adherent cells, Sci. Rep., 6, 21267, 10.1038/srep21267

Hermanowicz, 2014, AtomicJ: an open source software for analysis of force curves, Rev. Sci. Instrum., 85, 063703, 10.1063/1.4881683

Johnson, 1987

Jorba, 2019, Nonlinear elasticity of the lung extracellular microenvironment is regulated by macroscale tissue strain, Acta Biomater., 92, 265, 10.1016/j.actbio.2019.05.023

Jorba, 2017, Ageing and chronic intermittent hypoxia mimicking sleep apnea do not modify local brain tissue stiffness in healthy mice, J. Mech. Behav. Biomed. Mater., 71, 106, 10.1016/j.jmbbm.2017.03.001

Jorba, 2017, Probing micromechanical properties of the extracellular matrix of soft tissues by atomic force microscopy, J. Cell. Physiol., 232, 19, 10.1002/jcp.25420

Kuntz, 2014, Drosophila embryogenesis scales uniformly across temperature in developmentally diverse species, PLoS Genet., 10, e1004293, 10.1371/journal.pgen.1004293

Oliveira, 2019, Biomechanical response of lung epithelial cells to iron oxide and titanium dioxide nanoparticles, Front. Physiol., 10, 1047, 10.3389/fphys.2019.01047

Rickert, 2013, Labeling of single cells in the central nervous system of Drosophila melanogaster, J. Vis. Exp., 73, e50150

Rico Camps, 2006

Rico, 2005, Probing mechanical properties of living cells by atomic force microscopy with blunted pyramidal cantilever tips, Phys. Rev. E Stat. Nonlin. Soft Matter Phys., 72, 021914, 10.1103/PhysRevE.72.021914

Schillers, 2017, Standardized nanomechanical atomic force microscopy procedure (SNAP) for measuring soft and biological samples, Sci. Rep., 7, 5117, 10.1038/s41598-017-05383-0

Sirghi, 2006, Cleaning and hydrophilization of atomic force microscopy silicon probes, J. Phys. Chem. B, 110, 25975, 10.1021/jp063327g