Maternal high-protein diet modulates hepatic growth axis in weaning piglets by reprogramming the IGFBP-3 gene

Springer Science and Business Media LLC - Tập 59 Số 6 - Trang 2497-2506 - 2020
Rihua Cong1, Xiaoli Qu2, Hui Zhang1, Yongling Hu1, Silin Ye1, Demin Cai3,4, Xian Li1, Hao-Yu Liu5
1College of Veterinary Medicine, Northwest A and F University, Yangling, China
2School of Life Sciences, Zhengzhou University, Zhengzhou, China
3College of Animal Science and Technology, Yangzhou University, Yangzhou, China
4Department of Biochemistry and Molecular Medicine, University of California at Davis, Sacramento, USA
5Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden

Tóm tắt

Abstract Purpose The aim of this study was to investigate the effects of maternal high dietary protein intake on the hepatic growth axis in offspring. Methods Fourteen primiparous purebred Meishan sows were fed either a standard-protein (SP, n = 7) diet or a high-protein (HP, 150% of SP, n = 7) diet during pregnancy. Offspring (one male and one female per group, n = 14) on day 70 of the embryonic stage and on days 1, 35 and 180 after birth were selected, weighed and killed. Serum samples were analyzed for Tch, insulin and insulin-like growth factor-binding protein 3 (IGFBP-3) levels. Liver samples were analyzed for IGFBP-3 and IGF-I mRNA expression by qRT-PCR and for IGFBP-3, IGF1R and growth hormone receptor (GHR) protein expression by Western blotting. The underlying mechanism of IGFBP-3 regulation was determined by methylated DNA immunoprecipitation (MeDIP) and chromatin immunoprecipitation (ChIP). Results High-protein exposure resulted in significantly higher body and liver weights of piglets, and it increased their serum T3 and T4 levels at birth and/or at weaning. Furthermore, the IGFBP-3 protein content in the liver and serum was significantly reduced in the HP-exposed weaning piglets, whereas at the transcriptional level IGFBP-3 mRNA expression was downregulated in the livers of HP group piglets. Finally, DNA hypermethylation and higher enrichment of the histone repressive marks H3K27me3 and H3K9me3 were observed. Conclusions Taken together, these results suggest that a maternal high-protein diet during gestation epigenetically reprograms IGFBP-3 gene expression to modulate the hepatic growth axis in weaning piglets.

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Tập 59 Số 6

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