Mapping the four‐horned locus and testing the polled locus in three Chinese sheep breeds

Animal Genetics - Tập 47 Số 5 - Trang 623-627 - 2016
Xiaohong He1, Zhengkui Zhou1, Yun Pu1, Xiaofei Chen1, Yuehui Ma1, Lin Jiang1
1Key Laboratory for Farm Animal Genetic Resources and Utilization of the Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing, 100193 China

Tóm tắt

SummaryFour‐horned sheep are an ideal animal model for illuminating the genetic basis of horn development. The objective of this study was to locate the genetic region responsible for the four‐horned phenotype and to verify a previously reported polled locus in three Chinese breeds. A genome‐wide association study (GWAS) was performed using 34 two‐horned and 32 four‐horned sheep from three Chinese indigenous breeds: Altay, Mongolian and Sishui Fur sheep. The top two significant single nucleotide polymorphisms (SNPs) associated with the four‐horned phenotype were both located in a region spanning positions 132.6 to 132.7 Mb on sheep chromosome 2. Similar locations for the four‐horned trait were previously identified in Jacob, Navajo‐Churro, Damara and Sishui Fur sheep, suggesting a common genetic component underlying the four‐horned phenotype. The two identified SNPs were both downstream of the metaxin 2 (MTX2) gene and the HOXD gene cluster. For the top SNP—OAR2:g.132619300G>A—the strong associations of the AA and AG genotypes with the four‐horned phenotype and the GG genotype with the two‐horned phenotype indicated the dominant inheritance of the four‐horned trait. No significant SNPs for the polled phenotype were identified in the GWAS analysis, and a PCR analysis for the detection of the 1.8‐kb insertion associated with polled sheep in other breeds failed to verify the association with polledness in the three Chinese breeds. This study supports the hypothesis that two different loci are responsible for horn existence and number. This study contributes to the understanding of the molecular regulation of horn development and enriches the knowledge of qualitative traits in domestic animals.

Từ khóa


Tài liệu tham khảo

10.1093/hmg/5.7.945

Alderson L., 1992, Polycerate inheritance, Arkansans, 19, 177

10.1093/oxfordjournals.jhered.a105269

10.1111/j.1365-2052.2011.02271.x

Du L., 2011, Animal Genetic Resources in China: Sheep and Goats

Dýrmundsson Ó.R., 2005, Four–hornedness; a rare peculiarity still found in Icelandic sheep, The Icelandic Sheep Breeders of North America Newsletter, 9, 6

10.1038/091086b0

10.1086/338921

10.1111/age.12411

2015 T. Hadfield N. Cockett J.W. Kijas The polycerate trait in Jacob and Navajo‐Churro sheep may be located on ovine chromosome 2

10.1038/hdy.2009.109

10.1111/j.1365-294X.2011.05076.x

10.1038/nature12489

10.1371/journal.pbio.1001258

10.1111/age.12409

10.1093/bioinformatics/bts444

10.2527/2004.82102900x

10.1093/oxfordjournals.jhered.a023014

10.1038/ng1847

10.1038/srep21111

10.1038/091010c0

10.1017/S1014233909990411

10.1111/age.12309

Thông tin
Thông tin xuất bản

Animal Genetics

Tập 47 Số 5

623-627

Thông tin tác giả