Macromolecular Architecture in Eukaryotic Cells Visualized by Cryoelectron Tomography

American Association for the Advancement of Science (AAAS) - Tập 298 Số 5596 - Trang 1209-1213 - 2002
Ohad Medalia1, I.T. Weber1, Achilleas S. Frangakis1, Daniela Nicastro1, Günther Gerisch1, Wolfgang Baumeister1
1Max Planck Institute for Biochemistry, D-82152 Martinsried, Germany

Tóm tắt

Electron tomography of vitrified cells is a noninvasive three-dimensional imaging technique that opens up new vistas for exploring the supramolecular organization of the cytoplasm. We applied this technique to Dictyostelium cells, focusing on the actin cytoskeleton. In actin networks reconstructed without prior removal of membranes or extraction of soluble proteins, the cross-linking of individual microfilaments, their branching angles, and membrane attachment sites can be analyzed. At a resolution of 5 to 6 nanometers, single macromolecules with distinct shapes, such as the 26 S proteasome, can be identified in an unperturbed cellular environment.

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O.M. thanks the European Commission for a Marie Curie fellowship. I.W. was supported by a grant from the Deutsche Forschungsgemeinschaft (SFB 266 to G.G.). We thank J. Köhler for the movie and A. C. Steven for critical reading of the manuscript.

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American Association for the Advancement of Science (AAAS)

Tập 298 Số 5596

1209-1213

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