M1/70 attenuates blood-borne neutrophil oxidants, activation, and myofiber damage following stretch injury

Journal of Applied Physiology - Tập 95 Số 3 - Trang 969-976 - 2003
Stacey Brickson1, Lili Ji2, Kathleen Schell3, Ronke M. Olabisi3, Barbara St. Pierre Schneider3, Thomas M. Best3
1Department of Kinesiology, University of Wisconsin-Madison, Madison, WI 53706, USA. [email protected]
2University of Wisconsin
3University of Wisconsin, Madison

Tóm tắt

The purpose of this study was to determine the role of the CD11b-dependent respiratory burst in neutrophil oxidant generation and activation, interleukin-8 (IL-8) production, and myofiber damage after muscle stretch injury by using the monoclonal antibody M1/70 to block this pathway. Twelve male New Zealand White rabbits were randomly assigned to a treatment group: M1/70 ( n = 6), IgG isotype control ( n = 3), or saline control ( n = 3). After intravenous injection of the assigned agent under gas anesthesia, a standardized single-stretch injury was created in the right tibialis anterior, whereas the left tibialis anterior underwent a sham surgery. Blood-borne neutrophil oxidant generation and CD11b receptor density and plasma IL-8 levels were measured pre- and 24 h postinjury. Damage was assessed histologically at the hematoma site by counting torn myofibers. M1/70 group demonstrated decreased blood-borne neutrophil oxidant generation ( P < 0.05) and CD11b receptor density ( P < 0.05), an increase in plasma IL-8 concentration ( P < 0.01), and less torn myofibers ( P < 0.01) compared with IgG isotype or saline control groups. These data indicate that 1) CD11b-dependent respiratory burst is a major source of oxidants produced by the neutrophil, and that treatment with M1/70 2) attenuates neutrophil activation status, 3) increases plasma IL-8 concentration, and 4) minimizes myofiber damage 24 h postmuscle stretch injury.

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Tài liệu tham khảo

10.1177/036354658601400411

10.1046/j.1365-2567.1996.d01-784.x

Anderson DC, Miller LJ, Schmalstieg FC, Rothlein R, and Springer TA.Contributions of the Mac-1 glycoprotein family to adherence-dependent granulocyte functions: structure-function assessments employing subunit-specific monoclonal antibodies.J Immunol137: 15-27, 1986.

Ault KAand Springer TA.Cross-reaction of the rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells.J Immunol126: 359-364, 1981.

10.1016/0014-5793(92)80909-Z

Bar PR, Reijneveld JC, Wokke JHJ, Jacobs SCJM, and Bootsma AL.Muscle damage induced by exercise: nature, prevention and repair. In:Muscle Damage, edited by Salmons S. Oxford, UK: Oxford University Press, 1997, p. 1-27.

10.1152/jappl.1999.87.1.74

Best TM, Hasselman CT, and Garrett WE.Clinical aspects and basic science of muscle strain injuries.Basic Appl Myol4: 77-90, 1994.

10.1097/00005768-199802000-00005

10.1002/(SICI)1521-4141(199911)29:11<3419::AID-IMMU3419>3.0.CO;2-1

10.1016/S0022-5223(98)70249-1

10.1097/00005768-200112000-00006

Broaddus VC, Boylan AM, Hoeffel JM, Kim KJ, Sadick M, Chuntharapai A, and Hebert CA.Neutralization of IL-8 inhibits neutrophil influx in rabbit model of endotoxin-induced pleurisy.J Immunol152: 2690-2697, 1994.

10.1016/S0039-6060(97)90265-8

10.1016/0006-291X(89)92009-3

10.1002/bjs.1800830234

10.1007/s000110050208

Dana N, Styrt B, Griffin GD, Todd RF III, Klempner MS, and Arnaout MA.Two functional domains in the phagocyte membrane glycoprotein Mo1 identified with monoclonal antibodies.J Immunol137: 3259-3263, 1986.

10.1159/000157027

10.1084/jem.171.4.1155

Ding ZM, Babensee JE, Simon SI, Lu H, Perrard JL, Bullard DC, Dai XY, Bromley SK, Dustin ML, Entman ML, Smith CW, and Ballantyne CM.Relative contribution of LFA-1 and Mac-1 to neutrophil adhesion and migration.J Immunol163: 5029-5038, 1999.

Edwards SW.Regulation of neutrophil oxidant production. In:Calcium, Oxygen Radicals, and Cellular Damage, edited by Duncan CJ. Cambridge, UK: Cambridge University Press, 1991, p. 35-76.

10.1093/ptj/73.12.911

10.1172/JCI118009

10.1016/0046-8177(92)90317-V

10.1016/0167-4889(95)00169-7

10.1007/s004410051108

Friedland JS, Constantin D, Shaw TC, and Stylianou E.Regulation of interleukin-8 gene expression after phagocytosis of zymosan by human monocytic cells.J Leukoc Biol70: 447-454, 2001.

Halliwell Band Gutteridge JM.Free Radicals in Biology and Medicine(3rd ed.). New York: Oxford University Press, 1999.

10.1016/1357-4310(96)10042-3

Hashimoto S, Yasuhiro G, Matsumoto K, Takeshita I, and Horie T.N-acetylcysteine attenuates TNF-α-induced p38 MAP kinase activation and p38 MAP kinase-mediated IL-8 production by human pulmonary vascular endothelial cells.Br J Pharmacol133: 270-276, 2001.

10.2106/00004623-200205000-00022

10.4049/jimmunol.167.1.451

10.1152/ajpheart.1988.254.5.H823

Kuijpers TW, Tool AT, van der Schoot CE, Ginsel LA, Onderwater JJ, Roos D, and Verhoeven AJ.Membrane surface antigen expression on neutrophils: a reappraisal of the use of surface markers for neutrophil activation.Blood78: 1105-1111, 1991.

10.1034/j.1600-0609.2000.90198.x

10.1152/jappl.1994.77.4.1926

10.1165/ajrcmb.23.2.3853

10.1152/japplphysiol.00766.2002

Mishra DK, Friden J, Schmitz MC, and Lieber RL.Anti-inflammatory medication after muscle injury.J Bone Joint Surg.77-A: 1510-1519, 1995.

Na YJ, Jeon YJ, Suh JH, Kang JS, Yang KH, and Kim HM.Suppression of IL-8 gene expression by radicicol is mediated through the inhibition of ERK1/2 and p38 signaling and negative regulation of NF-kappaB and AP-1.Int Immunol1: 1877-1887, 2001.

Niess AM, Dickhuth HH, Northoff H, and Fehrenbackf E.Free radicals and oxidative stress in exercise-immunological aspects. In:Exercise Immunology Review, edited by Northoff H. Champaign, IL: Human Kinetics, 1999, p. 22-56.

O'Gorman MRand Corrochano V.Rapid whole-blood flow cytometry assay for diagnosis of chronic granulomatous disease.Clin Diagn Lab Immunol2: 227-232, 1995.

10.1007/s004210170012

10.1159/000028274

10.1083/jcb.112.4.749

Price TH, Beatty PG, and Corpuz SR.In vivo inhibition of neutrophil function in the rabbit using monoclonal antibody to CD18.J Immunol139: 4174-4177, 1987.

10.1016/0022-1759(95)00105-J

10.1073/pnas.95.17.10134

10.1084/jem.166.6.1685

Smet EG, Smet WD, Brys L, and Baetselier PCD.A monoclonal antibody recognizing the complement type 3 receptor (CD3) in the rabbit.Immunology59: 419-425, 1986.

10.1002/mus.10109

10.1016/S0168-8278(00)80219-6

10.1177/036354659302100205

10.1002/1097-0320(20010401)43:4<290::AID-CYTO1061>3.0.CO;2-X

10.1096/fasebj.13.13.1855

10.1152/jappl.1992.72.6.2168

10.1161/01.ATV.20.12.2553

Wilkinson JM, Galea-Lauri J, Sellars RA, and Boniface C.Identification and tissue distribution of rabbit leucocyte antigens recognized by monoclonal antibodies.Immunology76: 625-630, 1992.

Wozniak A, Betts WH, Murphy GA, and Rokicinski M.Interleukin-8 primes human neutrophils for enhanced superoxide anion production.Immunology79: 608-615, 1993.

10.1006/bbrc.1996.1691

Zerba E, Komorowski TE, and Faulkner J.Free radical injury to skeletal muscles of young, adult, and old mice.Am J Physiol Cell Physiol258: C429-C435, 1990.