Nhiệt độ thấp làm thay đổi thành phần lipid màng plasma và hoạt động ATPase của trái dứa trong quá trình phát triển bệnh đen lòng

Journal of bioenergetics - Tập 46 - Trang 59-69 - 2014
Yuchan Zhou1,2, Xiaoping Pan3, Hongxia Qu3, Steven J. R. Underhill1,2
1Queensland Alliance for Agriculture and Food Innovation, University of Queensland, St Lucia, Australia
2Faculty of Science, Education and Engineering, University of the Sunshine Coast, Sippy Downs, Australia
3Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China

Tóm tắt

Màng plasma (PM) đóng vai trò trung tâm trong việc kích hoạt các phản ứng ban đầu với tổn thương do lạnh và duy trì sự cân bằng tế bào. Việc đặc trưng phản ứng của lipid màng đối với nhiệt độ thấp có thể cung cấp thông tin quan trọng để xác định các yếu tố nguyên nhân sớm góp phần gây ra tổn thương do lạnh. Để làm điều này, thành phần lipid màng PM và hoạt động ATPase được đánh giá trong trái dứa (Ananas comosus) liên quan đến tác động của nhiệt độ thấp đến sự phát triển của bệnh đen lòng, một dạng tổn thương do lạnh. Nhiệt độ lạnh ở 10 °C đã gây ra sự phát triển của bệnh đen lòng song song với sự gia tăng rò rỉ điện giải. Hoạt động ATPase ở màng plasma giảm sau 1 tuần ở nhiệt độ thấp, tiếp theo là sự giảm thêm sau 2 tuần. Hoạt động enzyme không thay đổi trong quá trình bảo quản ở 25 °C. Sự mất mát phospholipid PM tổng cộng được tìm thấy trong quá trình lão hóa sau thu hoạch, nhưng có sự giảm nhiều hơn khi bảo quản ở 10 °C. Phosphatidylcholine và phosphatidylethanolamine là các loài phospholipid PM chủ yếu. Nhiệt độ thấp làm tăng mức độ axit phosphatidic nhưng giảm mức độ phosphatidylinositol. Cả hai loài phospholipid này không có sự thay đổi trong quá trình bảo quản ở 25 °C. Việc bảo quản sau thu hoạch ở cả hai nhiệt độ làm giảm mức độ C18:3 và C16:1, và tăng mức độ C18:1. Nhiệt độ thấp giảm mức độ C18:2 và tăng mức độ C14:0. Việc ứng dụng bên ngoài axit phosphatidic được phát hiện làm ức chế hoạt động ATPase của màng plasma trái dứa trong ống nghiệm. Việc sửa đổi thành phần lipid màng và ảnh hưởng của nó đối với tính chất chức năng của màng plasma ở nhiệt độ thấp đã được thảo luận liên quan đến vai trò của chúng trong sự phát triển bệnh đen lòng của trái dứa.

Từ khóa

#màng plasma #lipid #hoạt động ATPase #dứa #bệnh đen lòng #nhiệt độ thấp

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