Low-affinity penicillin-binding protein associated with beta-lactam resistance in Staphylococcus aureus

Journal of Bacteriology - Tập 158 Số 2 - Trang 513-516 - 1984
Barry J. Hartman, Alexander Tomasz

Tóm tắt

Methicillin resistance in Staphylococcus aureus has been associated with alterations in the penicillin-binding proteins (PBPs). An intriguing property of all methicillin-resistant staphylococci is the dependence of resistance on the pH value of the growth medium. Growth of such bacteria at pH 5.2 completely suppressed the expression of methicillin resistance. We have examined the PBP patterns of methicillin-resistant staphylococci grown at pH 7.0. We detected a high-molecular-weight PBP (PBP-2a; approximate size, 78,000 daltons) that was only present in the resistant bacteria but not in the isogenic sensitive strain. In cultures grown at pH 5.2, the extra PBP was not detectable.

Từ khóa


Tài liệu tham khảo

Bonner , W. M. , and R. A. Laskey . 1974 . A film detection method for tritium-labeled proteins and nucleic acids in polyacrylamide gels . Eur. J. Biochem. 46 : 83 - 88 .

Brown , D. F. J. , and P. E. Reynolds . 1980 . Intrinsic resistance to ,B-lactam antibiotics in Staphylococcus aureus . FEBS Lett. 122 : 275 - 278 .

Buchanan , C. E. , and J. L. Strominger . 1976 . Altered penicillinbinding components in penicillin-resistant mutants of Bacillus subtilis . Proc. Natl. Acad. Sci. U.S.A. 73 : 1816 - 1820 .

Centers for Disease Control. 1981 . Methicillin-resistant Staphylococcus aureus-United States . Morbid. Mortal. Weekly Rep. 30 : 557 - 559 .

Dougherty , T. J. , A. E. Koller , and A. Tomasz . 1980 . Penicillinbinding proteins of penicillin-sensitive and intrinsically resistant Neisseria gonorrhoeae . Antimicrob. Agents Chemother. 18 : 730 - 737 .

Fairbanks , G. T. , T. L. Steck , and D. H. F. Wallach . 1971 . Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane . Biochemistry 10 : 2606 - 2617 .

Fontana , R. , R. Cerini , P. Longoni , A. Grossato , and P. Canepari . 1983 . Identification of a streptococcal penicillin-binding protein that reacts very slowly with penicillin . J. Bacteriol. 155 : 1343 - 1350 .

Georgopapadakou , N. H. , S. A. Smith , and D. P. Bonner . 1982 . Penicillin-binding proteins in a Staphylococcus aureus strain resistant to specific P-lactam antibiotics . Antimicrob. Agents Chemother. 22 : 172 - 175 .

Hartman , B. , and A. Tomasz . 1981 . Altered penicillin-binding proteins in methicillin-resistant strains of Staphylococcus aureus . Antimicrob. Agents Chemother. 19 : 726 - 735 .

Hayes , M. V. , N. A. C. Curtis , A. W. Wyke , and J. B. Ward . 1981 . Decreased affinity of a penicillin binding protein for ,Blactam antibiotics in a clinical isolate of Staphylococcus aureus resistant to methicillin . FEMS Microbiol. Lett. 10 : 119 - 122 .

Laemmli , U. K. 1970 . Cleavage of structural proteins during the assembly of the head of bacteriophage T4 . Nature (London) 227 : 680 - 685 .

Mirelman , D. , Y. Nuchamowitz , and E. Rubinstein . 1981 . Insensitivity of peptidoglycan biosynthetic reactions to P-lactam antibiotics in a clinical isolate of Pseudomonas aeruginosa . Antimicrob. Agents Chemother. 19 : 687 - 695 .

O'Callaghan , C. H. , A. Morris , S. M. Kirby , and A. H. Shingler . 1972 . Novel method for detection of P-lactamases by using a chromogenic cephalosporin substrate . Antimicrob. Agents Chemother. 1 : 283 - 288 .

Rosegay , A. 1981 . High specific activity (Pheny1-3H) benzylpenicillin N-ethylpiperidine salt . J. Labelled Compd Radiopharm. 18 : 1337 - 1340 .

Sabath L. D. 1977. Chemical and physical factors influencing methicillin resistance of Staphylococcus aureus and Staphylococcus epidermidis. J. Antimicrob. Chemother. 3(Suppl. C):47-51.

Sabath , L. D. , S. J. Wallace , and D. A. Gerstein . 1972 . Suppression of intrinsic resistance to methicillin and other penicillins in Staphylococcus aureus . Antimicrob. Agents Chemother. 2 : 350 - 355 .

Saravolatz , L. D. , D. J. Pohlod , and L. M. Arking . 1982 . Community-acquired methicillin-resistant Staphylococcus aureus infections: a new source of nosocomial outbreaks . Ann. Intern. Med. 97 : 325 - 329 .

Schwarz , U. , K. Seeger , F. Wengenmayer , and H. Strecker . 1981 . Penicillin binding proteins of Escherichia coli identified with a 1251-derivative of ampicillin . FEMS Microbiol. Lett. 10 : 107 - 109 .

Spratt , B. G. 1978 . Escherichia coli resistance to 3-lactam antibiotics through a decrease in the affinity of a target for lethality . Nature (London) 274 : 713 - 715 .

Thompson , R. L. , I. Cabezudo , and R. P. Wenzel . 1982 . Epidemiology of nosocomial infections caused by methicillin-resistant Staphylococcus aureus . Ann. Intern. Med. 97 : 309 - 317 .

Zighelboim , S. , and A. Tomasz . 1980 . Penicillin-binding proteins of multiply antibiotic-resistant South African strains of Streptococcus pneumoniae . Antimicrob. Agents Chemother. 17 : 434 - 442 .