Wei Zhang1,2, Zhe Chen1,2, Xiaoxiao Song3, Jianmin Si1,2, Guping Tang3
1Department of Gastroenterology Institute of Clinical Medcine Sir Run Run Shaw Hospital Zhejiang University School of Medicine Hangzhou 310006
2Laboratory of Traditional Chinese Medicine & Gastroenterology Hangzhou 310006
3Department of Chemistry Zhejiang University Hangzhou 310028
Tóm tắt
The purpose of this study was to evaluate the characterization of a new cationic vector. Low generation polypropylenimine (DAB-8) was conjugated to β-cyclodextrin (β-CyD), and the chemical characters of the vector were investigated and confirmed by 1 H NMR, FT-IR, TGA, TEM, particle size, and zeta potential assay. The biological property was identified by MTT assay and gene transfer efficiency was performed in cell lines. The results showed that the new vector had low cytotoxicity and high transfection efficiency in vitro. This suggested that β-CyD-DAB-8 has a potential ability to act as a non-viral vector in gene delivery.
