Loofa Sponge as a Scaffold for the Culture of Human Hepatocyte Cell Line

Biotechnology Progress - Tập 19 Số 2 - Trang 522-527 - 2003
Jyh‐Ping Chen1, Sheng‐Chun Yu2, Brend Ray‐Sea Hsu3, Shin‐Huei Fu3, Hwai‐Shen Liu2
1Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung University, Kwei‐San, Taoyuan 333, Taiwan, R.O.C
2Department of Chemical Engineering, National Taiwan University, Taipei 106, Taiwan, R.O.C.
3Division of Endocrinology and Metabolism, Chang Gung Memorial Hospital, Kwei‐San, Taoyuan 333, Taiwan, R.O.C

Tóm tắt

AbstractLoofa sponge was investigated as a three‐dimensional scaffold for stationary and perfusion culture of human hepatoblastoma cell line C3A/HepG2. In stationary culture, C3A/HepG2 cells in loofa cubes showed higher α‐fetoprotein and albumin secretion rates than those in polyurethane foam (PU). To use loofa cylinders in a packed‐bed reactor, immobilization of C3A/HepG2 cells by recirculating medium at 26 mL/min (superficial velocity = 51.7 cm/min) resulted in a cell loading density of 5.15 × 107 cells/cm3‐loofa. This cell loading density is higher than values reported in the literature for packed‐bed reactor intended for bioartificial liver. During 9 days of perfusion culture in the reactor, immobilized C3A/HepG2 showed steady synthesis of albumin with an average synthesis rate at 42.2 μg/106 cells/day. These experimental results and observations by SEM suggested that loofa sponge is a suitable scaffold for high‐density culture of human hepatocyte cell line and the immobilized cells could express high levels of liver‐specific functions.

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Biotechnology Progress

Tập 19 Số 2

522-527

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