Localized injury in cardiomyocyte network: a new experimental model of ischemia-reperfusion arrhythmias

American Journal of Physiology - Heart and Circulatory Physiology - Tập 280 Số 4 - Trang H1905-H1915 - 2001
Ara Arutunyan1, Daniel R. Webster2, Luther Swift1, Narine Sarvazyan1
1Department of Physiology and
2Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430

Tóm tắt

We developed a new experimental approach to study the effects of local injury in a multicellular preparation and tested the ability of the method to induce reperfusion arrhythmias in cardiomyocyte monolayers. A small region of injury was created using geometrically defined flows of control and ischemia-like solutions. Calcium transients were acquired simultaneously from injured, control, and border zone cells using fluo 4. Superfusion with the injury solution rapidly diminished the amplitude of calcium transients within the injury zone, followed by cessation of cell beating. Reperfusion caused an immediate tachyarrhythmic response in ∼17% of experiments, with a wave front propagating from a single cell or small cell cluster within the former injury zone. Inclusion of a gap junction uncoupler (1 mM heptanol) in the injury solution narrowed the functional border and sharply increased the number of ectopic foci and the incidence of reperfusion arrhythmias. The model holds a potential to reveal both micro- and macroscopic features of propagation, conduction, and cell coupling in the normal and diseased myocardium and to serve as a new tool to test antiarrhythmic protocols in vitro.

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American Journal of Physiology - Heart and Circulatory Physiology

Tập 280 Số 4

H1905-H1915

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