LncRNAs and their regulatory networks in breast muscle tissue of Chinese Gushi chickens during late postnatal development

Springer Science and Business Media LLC - Tập 22 Số 1 - 2021
Yuanfang Li1, Wenjiao Jin1, Bin Zhai1, Yi Chen1, Guoxi Li1, Yanhua Zhang1, Yujie Guo1, Guirong Sun1, Ruili Han1, Zhuanjian Li1, Hong Li1, Yadong Tian1, Xiaojun Liu1, Xiangtao Kang2
1College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, China
2Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Henan Agricultural University, Zhengzhou 450002, China

Tóm tắt

AbstractBackgroundChicken skeletal muscle is an important economic product. The late stages of chicken development constitute the main period that affects meat production. LncRNAs play important roles in controlling the epigenetic process of growth and development. However, studies on the role of lncRNAs in the late stages of chicken breast muscle development are still lacking. In this study, to investigate the expression characteristics of lncRNAs during chicken muscle development, 12 cDNA libraries were constructed from Gushi chicken breast muscle samples from 6-, 14-, 22-, and 30-week-old chickens.ResultsA total of 1252 new lncRNAs and 1376 annotated lncRNAs were identified. Furthermore, 53, 61, 50, 153, 117, and 78 DE-lncRNAs were found in theW14vs.W6, W22vs.W14, W22vs.W6, W30vs.W6, W30vs.W14, andW30vs.W22comparison groups, respectively. After GO enrichment analysis of the DE-lncRNAs, several muscle development-related GO terms were found in theW22vs.W14comparison group. Moreover, it was found that the MAPK signaling pathway was one of the most frequently enriched pathways in the different comparison groups. In addition, 12 common target DE-miRNAs of DE-lncRNAs were found in different comparison groups, some of which were muscle-specific miRNAs, such as gga-miR-206, gga-miR-1a-3p, and miR-133a-3p. Interestingly, the precursors of four newly identified miRNAs were found to be homologous to lncRNAs. Additionally, we found some ceRNA networks associated with muscle development-related GO terms. For example, the ceRNA networks contained theDYNLL2gene with 12 lncRNAs that targeted 2 miRNAs. We also constructed PPI networks, such asIGF-I-EGFandFZD6-WNT11.ConclusionsThis study revealed, for the first time, the dynamic changes in lncRNA expression in Gushi chicken breast muscle at different periods and revealed that the MAPK signaling pathway plays a vital role in muscle development. Furthermore,MEF2Cand its target lncRNA may be involved in muscle regulation through the MAPK signaling pathway. This research provided valuable resources for elucidating posttranscriptional regulatory mechanisms to promote the development of chicken breast muscles after hatching.

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Tập 22 Số 1

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