Lipid peroxidation as a possible secondary mechanism of sterigmatocystin toxicity
Tóm tắt
Sterigmatocystin (Stg), a major secondary metabolite of Aspergillus versicolor and A. nidulans, is the precursor of aflatoxin B1 In this study, male albino rats were treated with Stg-contaminated diet for 30 days, resulting in reduced levels of glutathione, ascorbic acid, and tocopherol. The activity of catalase in liver was reduced, whereas an increase in the activities of superoxide dismutase and glutathione peroxidase was observed. The levels of cytochrome P450, cytochrome b 5, cyto-chrome b 5 reductase, cytochrome c reductase, hydroxyl radical, and hydrogen peroxide formation significantly increased in the Stg-treated rat liver microsomes. Hepatic parenchymal cell injury, necrosis, and Kupffer cells proliferation were noticed in histological sections of liver from animals treated with Stg. Overall results suggest that generation of free radicals imposes depletion of antioxidants. This led to enhanced lipid peroxidation. The observed elevation of hepatic thiobarbituric acid reactive substances appears to originate mainly from the damaged Kupffer cells. As a result, elevated levels of serum marker enzymes were also observed.
Từ khóa
Tài liệu tham khảo
Atroshi F, 2000, J Pharm Sci, 3, 281
Cederbaum AI , Cohen G . In: Packer L (Ed.), Methods in Enzymology, vol. 105. Academic Press, San Diego , 1984, pp. 516–522.
Hilderbrandt AG , Roots I , Tjoe M , Heinemeyer G . In: Fleischer S , Packer L (Eds.), Methods in Enzymology, vol. 52. Academic Press, San Diego , 1978, pp. 342–350.
Omaye ST, 1971, Methods Enzymol, 62, 1
Buege JA , Aust SD . In: Fleischer S , Packaer L (Eds.), Methods in Enzymology, vol. 52. Academic Press, San Diego , 1978, pp. 302–310.
Indirani N, 1977, Biochem Biophys Acta, 483, 57
King J . Transferases. Practical Clinical Enzymology. Van Nostrand, New Jersey , 363-363 pp.
King J . Transferases. Practical Clinical Enzymology. Van Nostrand, New Jersey , 106-106 pp.
King J . Phosphatases. Practical Clinical Enzymology. Van Nostrand, New Jersey , 191-191 pp.
Parke DV, 1979, Proc Nutr Soc Aust, 4, 61
Eaton DL, 1994, Microbial Toxins in Foods and Feeds: Molecular Modes of Action, 275
Rafat SA, 1997, Indian J Exp Biol, 35, 46
Rose RC, 1989, Am J Physiol, 256, F52
Scholich H, 1989, Biochim Biophys Acta, 1001, 49
Cheesman KH, 1993, DNA and Free Radicals, 109
Karagezyan MK, 1995, Doklady Akademii Nauk, 341, 259
Sadani G, 1994, Indian J Exp Biol, 32, 354