Linking rhizosphere microbiome composition of wild and domesticated Phaseolus vulgaris to genotypic and root phenotypic traits

ISME Journal - Tập 11 Số 10 - Trang 2244-2257 - 2017
Juan E. Pérez‐Jaramillo1,2, Víctor J. Carrión1, Mirte Bosse3, Luiz F V Ferrão4, Mattias de Hollander1, Antônio Augusto Franco Garcia4, Camilo A. Ramírez5, Rodrigo Mendes6, Jos M. Raaijmakers1,2
1Department of Microbial Ecology, Netherlands Institute of Ecology (NIOO-KNAW), Wageningen, The Netherlands
2Institute of Biology, Leiden University, Leiden, the Netherlands
3Department of Animal Ecology, Netherlands Institute of Ecology (NIOO-KNAW), Wageningen, The Netherlands
4Department of Genetics, Escola Superior de Agricultura Luiz de Queiroz (ESALQ), Universidade de São Paulo (USP) , Piracicaba, São Paulo, Brazil
5Institute of Biology, University of Antioquia, Medellín, Colombia
6Laboratory of Environmental Microbiology, Brazilian Agricultural Research Corporation, Embrapa Environment, Jaguariúna, Brazil

Tóm tắt

Abstract Plant domestication was a pivotal accomplishment in human history, but also led to a reduction in genetic diversity of crop species compared to their wild ancestors. How this reduced genetic diversity affected plant–microbe interactions belowground is largely unknown. Here, we investigated the genetic relatedness, root phenotypic traits and rhizobacterial community composition of modern and wild accessions of common bean (Phaseolus vulgaris) grown in agricultural soil from the highlands of Colombia, one of the centers of common bean diversification. Diversity Array Technology-based genotyping and phenotyping of local common bean accessions showed significant genetic and root architectural differences between wild and modern accessions, with a higher specific root length for the wild accessions. Canonical Correspondence Analysis indicated that the divergence in rhizobacterial community composition between wild and modern bean accessions is associated with differences in specific root length. Along the bean genotypic trajectory, going from wild to modern, we observed a gradual decrease in relative abundance of Bacteroidetes, mainly Chitinophagaceae and Cytophagaceae, and an increase in relative abundance of Actinobacteria and Proteobacteria, in particular Nocardioidaceae and Rhizobiaceae, respectively. Collectively, these results establish a link between common bean domestication, specific root morphological traits and rhizobacterial community assembly.

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