Ligninolytic activity of tropical rainforest basidiomycetes

World Journal of Microbiology and Biotechnology - Tập 16 - Trang 889-893 - 2000
Luci Kimie Okino1, Kátia Maria Gomes Machado2, Carla Fabris1, Vera Lúcia Ramos Bononi1
1Seção de Micologia e Liquenologia, Instituto de Botânica, São Paulo, SP
2Fundação Centro Tecnológico de Minas Gerais – CETEC, Belo Horizonte, MG, Brasil

Tóm tắt

A total of 116 strains of Brazilian tropical rainforest basidiomycetes were evaluated in terms of their ability to oxidize the dye rhemazol brilliant blue R (RBBR) and guaiacol. Laccase and peroxidase activities were detected by the drop test using solutions of α-naphthol and pyrogallol, respectively. RBBR and guaiacol oxidation occurred in 96.6 and 87.1% of the strains tested, respectively. One hundred strains oxidized both substrates. In the drop test, most strains presented laccase (96.6%) and peroxidase (92.2%) activity. The quick screening method used here can be useful to identify ligninolytic fungal strains to be used in various biotechnological applications.

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