Lateral diffusion of PDGF β-receptors in human fibroblasts

Bioscience Reports - Tập 11 Số 1 - Trang 43-52 - 1991
Pia Ljungquist-Höddelius1, Margareta Lirvall1, Åke Wasteson1, Karl‐Eric Magnusson2
1Department of Cell Biology, Faculty of Heath Sciences, University of Linköping, S-581 85 Linköping, Sweden
2Department of Medical Microbiology, Faculty of Heath Sciences, University of Linköping, S-581 85 Linköping, Sweden

Tóm tắt

When platelet-derived growth factor (PDGF) binds to its receptors a number of biochemical reactions are elicited in the cell. Several models have been presented for the effects of ligand-induced receptor conformation and aggregation on signal transduction but little is known about the direct effects on receptor diffusion. This study concerns the lateral mobility of PDGF receptors in fibroblasts. It was assessed with fluorescence recovery after photobleaching (FRAP), using rhodaminated receptor antibodies or Fab-fragments of the antibody as ligands. The aims of the investigation were: (a) to compare the lateral mobility of membrane receptors of human fibroblasts labelled with either antibodies against the PDGF receptor or Fab-fragments of the same antibodies, and (b) to study the effects of serum or PDGF on the mobility of the receptors. Human foreskin fibroblasts (AG 1523) were grown on coverslips either under standard or under serum-free conditions yielding “normal” and “starved” cells, respectively. Two parameters of the diffusion were evaluated; the diffusion coefficient (D) and the mobile fraction (R) of the receptors. We found that normal fibroblasts had a smaller diffusion coefficient and a lower mobile fraction compared to starved cells using antibodies for receptor labelling. The addition of PDGF, just before the measurement, increased the D and R for normal cells, while starved cells, showing higher initial values, displayed slightly reduced values of D and R. After the addition of serum, D increased and R remained low for normal cells, whereas for starved cells both D and R increased to upper limits of 11.0×10−10 cm2s−1 and >90% respectively. In general, the D and R values, both in normal and starved cells, were higher for cells labelled with Fab-fragments than for antibody-labelled cells. The results are discussed in relation to the natural complexity of the receptor, and how PDGF, serum, antibodies and Fab-fragments might interfere with receptor structure, aggregation state and membrane diffusion characteristics.

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Tài liệu tham khảo

Axelrod, D., Koppel, D. E., Schlessinger, J., Elson, E. and Webb, W. W. (1976)Biophys. J. 16:1055?1069.

Bockus, B. J. and Stiles, C. D. (1984)Exp. Cell. Res. 153:186?197.

Bowen-Pope, D. F. and Ross, R. (1984)Clin. Endocrin. Metab. 13:191?201.

Callahan, M., Cochran, B. H. and Stiles, C. D. (1985)Growth Factors in Biology and Medicine. In:Ciba Foundation Symposium 116:87?97.

Claesson-Welsh, L., Eriksson, A., Morén, A., Severinsson, L., Ek, B., Östman, A., Betsholtz, C. and Heldin, C.-H. (1989)Mol. Cell. Biol.,8:3476?3486.

Deuel, T. F., Senior, R. M., Huang, J. S. and Griffins, G. L. (1982)J. Clin. Invest. 69:1046?1049.

Ek, B. and Heldin, C.-H. (1982)J. Biol. Chem. 257:10486?10492.

Fukuo, K., Morimoto, S., Kaji, K., Koh, E., Hironaka, T., Morita, R., Kim, S. and Onishi, T. (1989)Cell calcium. 10:29?35.

Hammacher, A., Mellström, K., Heldin, C.-H. and Westermark, B. (1989)EMBO J,8:9, 2489?2495.

Harlan, E. I. and Daniel, T. O. (1987)Proc. Natl. Acad. Sci. USA. 84:1950?1954.

Heldin, C.-H., Wasteson, Å. and Westermark, B. (1985)Mol. Cell. Endocrinol.,39:169?187

Heldin, C.-H., Bäckström, G., Östman, A., Hammacher, A., Rönnstrand, L., Rubin, K., Nistér, M. and Westermark, B. (1988)EMBO J,7:1387?1393.

Heldin, C.-H., Johnsson, A., Wennergren, S., Wernstedt, C., Betsholtz, C. and Westermark, B. (1986)Nature,319:511?514.

Hudson, L. and Hay, F. C. (1980) In:Practical Immunology. Blackwell Scientific Publications. Osney Mead, Oxford, 191?196.

Jacobson, K., Derzko, Z., Wu, E. S., Hou, Y. and Poste, G. (1976)J. Supramol. Struct. 5:565?576.

Ljungquist, P., Wasteson, Å. and Magnusson, K.-E. (1989)Biosci Reps. 9:63?73.

Magnusson, K. E., Sundkvist, T. and Johansson, B. (1987)Eur Biophys J. 15:141?45.

Mellström, K., Höglund, A.-S., Nistér, M., Heldin, C.-H., Westermark, B. and Lindberg, U. (1983)J. Musc. Res Cell. Mot. 4:589?609.

Moolenaar, W. H., Defize, L. H. K. and De Laat, S. W. (1986).Calcium and the Cell. In:Ciba Foundation Symposium, Wiley, Chichester,122:212?231.

Peters, R., Peters, J., Tews, L. H. and Bähr, W. (1974)Biochem. Biophys. Acta. 367:282?294.

Peters, R. (1981)Cell. Biol. Int Reports 5:733?760.

Pledger, W. J. and Stiles, C. D. (1977)Proc. Natl. Acad. Sci. USA 74:4481?4485.

Rees, A. R., Gregoriou, M., Johnson, P. and Garland, P. (1984)EMBO J. 3: 1843?1847.

Rönnstrand, L., Beckmann, M. P., Faulders, B., Östman, A., Ek, B. and Heldin, C.-H. (1987)J. Biol. Chem. 262:2929?2932.

Rönnstrand, L., Terracio, L., Claesson-Welsh, L., Heldin, C. H. and Rubin, C. (1988)J. Biol. Chem. 263:10429?10435.

Ross, R. (1989)The Lancet,27:1179?1182.

Schlessinger, J., Axelrod, D., Koppel, D. E., Webb, W. W. and Elson, E. L. (1977)Science 195:307?309.

Schlessinger, J., Elson, E. L., Webb, W. W. and Metzger, H. (1976)Nature.264: 550?552.

Schlessinger, J., Koppel, D. E., Axelrod, D., Jacobson, K., Webb, W. W. and Elson, E. L. (1976)Proc. Natl. Acad. Sci. USA 73:2409?2413.

Schreiber, A. B., Liberman, T. A., Lax, I., Yarden, Y. and Schlessinger, J. (1983)J. Biol. Chem. 258:846?853.

Stiles, C. D., Capone, G. T., Scher, C. D., Antoniades, H. N., Van Wyk, J. J. and Pledger, W. J. (1979)Proc. Natl. Acad. Sci. USA 76:1279?1283.

Stroobrant, P. and Waterfield, M. D. (1984)EMBO J 2:2963?2967.

Vaz, W. L. C., Goodsaid-Zaldunodo, F. and Jacobson, K. (1984)FEBS Lett. 174:199?207.

Westermark, B. and Heldin, C.-H. (1985)J. Cell. Physiol. 124:43?48.

Westermark, B., Heldin, C.-H., Ek, B., Johnsson, A., Mellström, K., Nistér, K. and Wasteson, Å. (1983) In:Growth and Maturation Factors. Guroff G. (ed) Wiley, New York, 73?114.

Westermark, B., Johnsson, A., Paulsson, Y., Betsholtz, C., Betsholtz, C., Heldin, C.-H., Herlyn, M., Radeck, U. and Koprowski, H. (1986)Proc. Natl. Acad. Sci. USA,87:7197?7200.

Williams, L. T. (1989)Science 243:1564?1570.

Williams, L. T. (1983)J. Clin. Invest. 72:1759?1763.

Yarden, Y. and Shlessinger, J. (1985)Growth Factors in Biology and Medicine In:Ciba Foundation Symposium 116: 23?45.

Yarden, Y., Gabbay, M. and Schlessinger, J. (1981)Biochem. Biophys. Acta. 674:188?203.

Zidovetzki, R., Yarden, Y., Schlessinger, J. and Tovin, T. M. (1981)Proc. Natl. Acad. Sci. USA,78:6981?6985.

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Bioscience Reports

Tập 11 Số 1

43-52

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