Laminin isoforms in human embryonic stem cells: synthesis, receptor usage and growth support

Journal of Cellular and Molecular Medicine - Tập 13 Số 8b - Trang 2622-2633 - 2009
Sanna Vuoristo1,2, Ismo Virtanen3, Minna Takkunen3, Jaan Palgi1, Yamato Kikkawa4, Patricia Rousselle5, Kiyotoshi Sekiguchi6, Timo Tuuri1, Timo Otonkoski1,2
1Biomedicum Stem Cell Center, University of Helsinki, Helsinki, Finland
2Hospital for Children and Adolescents, University of Helsinki, Helsinki, Finland
3Institute of Biomedicine/Anatomy, University of Helsinki, Helsinki, Finland
4Laboratory of Clinical Biochemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, Japan
5Institut de Biologie et Chimie des Protéines, Unité Mixte de Recherche, Institut Fédératif de Recherche BioSciences Lyon-Gerland, Lyon, France
6Laboratory of Extracellular Matrix Biochemistry, Institute for Protein Research, Osaka University, Suita, Osaka, Japan

Tóm tắt

AbstractTo reveal the functional intrinsic niche of human embryonic stem cells (hESC) we examined the production of basement membrane (BM) proteins and the presence of their receptors in feeder‐free cell culture conditions. In addition, we investigated binding of hESCs to purified human BM proteins and identified the receptors mediating these contacts. Also, we tested whether purified human laminin (Lm) isoforms have a role in hESC self‐renewal and growth in short‐term cultures. The results show that hESCs synthesize Lm α1 and Lm α5 chains together with Lm β1 and γ1 chains suggesting the production of Lms‐111 and ‐511 into the culture medium and deposits on cells. hESCs contain functionally important integrin (Int) subunits, Int β1, α3, α6, α5, β5 and αV, as well as the Lm α5 receptor, Lutheran (Lu) glycoprotein and its truncated form, basal cell adhesion molecule (B‐CAM). In cell adhesion experiments, Int β1 was crucial for adhesion to most of the purified human BM proteins. Lu/B‐CAM mediated adhesion to Lm‐511 together with Int α3β1, and was essential for the adhesion of hESCs to embryonic feeder cells. Adhesion to Lm‐411 was mediated by Int α6β1. Lm‐511 supported hESC growth in defined medium equally well as Matrigel. These results provide consequential information of the biological role of BM in hESCs, warranting further investigation of BM biology of human pluripotent stem cells.

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Tài liệu tham khảo

10.1038/nrc1094

10.1007/s00109-007-0182-5

10.1146/annurev.cellbio.20.010403.094555

10.1016/S0955-0674(96)80112-8

10.1006/excr.2000.5042

10.1083/jcb.144.1.151

10.1242/dev.01112

10.1096/fasebj.7.14.8224605

10.1016/S0015-0282(01)01833-7

10.1083/jcb.115.3.843

10.1083/jcb.130.1.79

10.1074/jbc.M208731200

10.1152/ajpcell.00285.2005

10.1038/nbt1001-971

10.1038/nbt1177

10.1038/nature06027

10.1083/jcb.153.4.811

10.1038/ncb1660

10.1634/stemcells.2007-1122

10.1186/1471-213X-6-40

10.1006/excr.2000.4883

10.1074/jbc.M010155200

10.1182/blood.V76.3.570.570

10.1177/002215540205000813

10.1369/jhc.6A6958.2006

10.1007/s00125-008-0997-9

10.1002/ijc.2910200102

10.1016/S0945-053X(02)00052-5

10.1083/jcb.112.5.1031

10.1007/s00418-008-0443-6

10.1080/03008200500344074

10.1038/nature04957

10.1126/science.290.5490.328

10.1007/s00441-007-0503-6

10.1007/s12015-006-0047-2

10.2217/17460751.3.3.365

10.1634/stemcells.2007-0389

10.1016/j.ydbio.2003.12.034

10.1093/molehr/gal091

10.1634/stemcells.2008-0365

10.1016/S1534-5807(03)00128-X

10.1083/jcb.200203073

10.1634/stemcells.2008-0291

10.1093/molehr/8.3.237

10.1016/j.bbrc.2008.07.111

10.1074/jbc.M611706200

10.1152/ajprenal.00315.2007

10.1073/pnas.85.5.1544

10.1083/jcb.114.3.567

10.1182/blood-2004-01-0396

10.1091/mbc.1.10.731

Virtanen I, 1997, Distinct changes in the laminin composition of basement membranes in human seminiferous tubules during development and degeneration, Am J Pathol, 150, 1421

Wewer UM, 1997, Extrasynaptic location of laminin beta 2 chain in developing and adult human skeletal muscle, Am J Pathol, 151, 621

10.1177/002215540104900605

10.1073/pnas.81.7.2172

10.1182/blood.V73.5.1235.1235

10.1073/pnas.81.1.224

10.1083/jcb.105.4.1873

10.4049/jimmunol.132.6.3011

10.1083/jcb.109.2.877

10.1016/S0021-9258(18)45436-1

Yamada KM, 1990, Monoclonal antibody and synthetic peptide inhibitors of human tumor cell migration, Cancer Res, 50, 4485

10.1002/ijc.2910430628

10.1182/blood.V72.5.1478.1478

10.1083/jcb.111.4.1593

10.1242/jcs.105.1.101

10.1016/S0070-2153(08)60693-6

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Journal of Cellular and Molecular Medicine

Tập 13 Số 8b

2622-2633

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