Lactate dehydrogenase isozyme patterns of fish

Wiley - Tập 159 Số 3 - Trang 319-332 - 1965
Clement L. Markert1,2, Ilse Faulhaber1,2
1Bermuda Biological Station, St. George's West, Bermuda
2Department of Biology, The Johns Hopkins University, Baltimore, Maryland

Tóm tắt

AbstractRepresentatives of 30 species of fish were examined for their content of LDH isozymes. One major isozyme system was found in all fish. In addition, two minor systems restricted to eyes and to gonads were found in many fish. Fish may be classified into four groups on the basis of their possession of one, two, three, or five major isozymes of LDH. The major isozyme patterns can be attributed to the polymers of two protein subunits under the control of two genes, as in mammals and birds, but the variety of heteropolymers produced varies in different species. The fluke and other flatfish are exceptional in that they synthesize only one kind of subunit which polymerizes to form a single variety of LDH. All other fish produced at least two major isozymes presumably the homopolymers of A and B subunits. The three‐isozyme fish produce, in addition, a single heteropolymer. A few fish produce three heteropolymers and two homopolymers to produce the characteristic pattern of five isozymes seen in mammals.In populations of the whiting, which has five major isozymes, two mutant alleles at the B locus were discovered. Each of these alleles produced polypeptides with characteristic electrophoretic mobilities. Consequently, heterozygous individuals contained more than 15 isozymes.A variety of minor isozymes that appear on zymograms may represent modifications of the major isozymes. However, the minor isozyme systems of the gonad and the eye seem to be genetically distinct molecular systems, separate from and in addition to the major isozyme system.

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Tài liệu tham khảo

10.1002/jez.1401560202

10.1111/j.1471-4159.1963.tb08931.x

10.1126/science.141.3581.642

10.1126/science.136.3520.962

Daugherty Wayne1965Personal communication.

10.1016/0006-3002(63)90476-1

10.1126/science.139.3555.602

10.1016/0003-9861(65)90298-5

10.1126/science.148.3668.391

Henderson Nanine S.1965Thesis. The Johns Hopkins University Baltimore Maryland.

Kaplan N. O., 1963, Symposium on multiple forms of enzymes and control mechanisms. I. Multiple forms of enzymes, Bacteriol. Rev., 27, 155, 10.1128/br.27.2.155-169.1963

10.1111/j.1749-6632.1961.tb35567.x

10.1126/science.131.3398.392

10.1126/science.145.3632.595

10.1016/B978-0-12-395555-5.50009-8

10.1126/science.140.3573.1329

Markert C. L., 1964, Hybrid isozymes of lactate dehydrogenase, Abstracts of 6th International Congress of Biochemistry, 320

Markert C. L., 1965, The Harvey Lectures, 187

10.1002/jez.1401590203

10.1126/science.142.3595.1075

10.1126/science.130.3377.711

10.1073/pnas.50.2.211

10.1042/bj0610629

10.1085/jgp.45.3.553

Wieland T., 1959, über die Verschiedenheit Milchsäuredehydrogenasen. IV. Quantitative Ermittlung einiger Enzymverteilungsmuster Vergleichende Betrachtung bei verschiedenen Wirbeltierklassen, Biochem. Z., 332, 1

10.1111/j.1749-6632.1964.tb14209.x

Wilson A. C., 1964, Taxonomic Biochemistry and Serology, 321

Wilson A. C., 1964, Evolution of lactic dehydrogenases, Fed. Proc., 23, 1258

10.1126/science.142.3597.1303

10.1126/science.144.3624.1353

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Wiley

Tập 159 Số 3

319-332

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