Karyomegaly of tubular cells as early stage marker of the nephrotoxicity induced by ochratoxin A in rats
Tóm tắt
Cases of karyomegaly were described by Sclare30 and by Mihatch31 in patients affected with tubular-interstitial nephropathy. The Karyomegalic cells showed enlarged nuclei with accumulation of genetic material. No aetiology was suggested.
Our study of rats experimentally intoxicated by ochratoxin A, a well-known nephrotoxic compound, indicates the presence of karyomegaly with alteration of the tubular tissue. In control animals no karyomegalic cells were detected. These observations suggest that karyomegaly with megacytosis may be caused by the nephrotoxic ochratoxin A in the kidney.
In addition abnormal mitosis together with karyomegalic cells were observed at an earlier stage of the intoxication (30 days) suggesting possible regeneration if the OTA insults are stopped.
After 90 days of treatment, the degeneration increased and only karyomegalic and apoptotic-like cells were observed indicating that the regeneration no longer occurs and that the degeneration becomes irreversible.
Từ khóa
Tài liệu tham khảo
Bauer J, 1987, Z. Veterinärmed. B, 34, 613
Krogh P, 1987, Mycotoxin in food, 97
Kuiper-Goodman T, 1989, Biomed. Environ. Sci, 2, 179
CreppyEE, 1993, Human ochratoxicosis and its pathologies, INSERM/John Libbey EUROTEXT, 147
BachaH et al. In: Creppy EE , Castegnaro M , Dirheimer G (Eds.). Human ochratoxicosis and its pathologies, INSERM/John Libbey EUROTEXT, Paris , 1993; pp 111-121.
Krogh P, 1974, Acta Pathol. Microbiol. Scand., section A, Suppl, 246, 1
Boorman G. NTP technical Report on the Toxicology and Carcinogenesis Studies of ochratoxin A (CAS No 303-47-9) In F 344/N Rats (Gavage Studies), US. Department of Health and Human Services, National Institutes of Health, Research Triangle Park, NC, NIH Publication 89/ 2813 1989.
Krogh P, 1977, Acta. Pathol. Microbiol. Scand, 85, 238
Chernozemsky IN, 1977, Bulgaria J. Cancer, 19, 1
Achour A, 1993, Human ochratoxicosis and its pathologies, 227
Krogh P, 1985, Acta Pathol. Microbiol. Scand. Sect. B, 85, 238
Maaroufi K, 1994, Archives de l'Institut Pasteur, 71, 21
Baudrimont I. Variation de la néphrotoxicitéde l'ochratoxine A sous l'effet de facteurs métaboliques et non métaboliques. PhD Thesis (No 2) 1995, University Bordeaux 2, France.
Mantle PG, 1991, Mycotoxins, Endemic Nephropathy and Urinary tract tumours, IARC Scientific Publications, 119
Hard GC, 1991, Mycotoxins, Endemic Nephropathy and Urinary tract tumours, IARC Scientific Publications, 113
Gad SC , Neil CS . Statistic for toxicologists. In: Principles and Methods of Toxicology, 1982; pp 273-279.
Harwig J, 1983, Ochratoxins, Handbook of Food-borne Diseases of Biological Origin, 193
Mihatsch MJ, 1979, Clin. Nephrol, 12, 54