Jacalin: A new laboratory tool in immunochemistry and cellular immunology

Journal of Clinical Laboratory Analysis - Tập 3 Số 4 - Trang 244-251 - 1989
Pièrre Aucouturier1, Nathalie Pineau1, Jean‐Christophe Brugier1, Edith Mihaesco2, Françoise Duarte1, F Skvaril3, Jean‐Louis Preud'homme1
1Laboratory of Immunology and Immunopathology (CNRS URA 1172), University Hospital, Poitiers, France
2Laboratory of Immunochemistry and Immunopathology, INSERM U 108, Hôpital Saint-Louis, Paris, France.
3Institute for Clinical and Experimental Cancer Research, Bern, Switzerland

Tóm tắt

AbstractIn recent years, a new lectin—jacalin—has raised the interest of immunologists because of its original properties with respect to human immunoglobulins and lymphocytes. Its structure and carbohydrate binding specifity are now well documented, and it can be purified easily from jackfruit seeds by ion exchange or affinity chromatography. The binding and precipitating specificities of jacalin with heavy chains of human immunoglobulins allow its use as a diagnostic (IgA subclass typing) and preparative tool (purification of IgA and IgD, removal of IgA from biologic samples and preparations). Other possible applications of jacalin's binding properties also can be envisaged. In addition, the lectin displays a mitogenic activity specific for human CD4 T‐lymphocytes; consequently, the proliferative response induced by jacalin appears to represent a new and interesting assay for a functional study of CD4 cells, with obvious applications in primary and acquired, especially AIDS, immune deficiency states.

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Journal of Clinical Laboratory Analysis

Tập 3 Số 4

244-251

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