Isothiocyanate‐rich Moringa oleifera extract reduces weight gain, insulin resistance, and hepatic gluconeogenesis in mice

Molecular Nutrition and Food Research - Tập 59 Số 6 - Trang 1013-1024 - 2015
Carrie Waterman1, Patricio Rojas‐Silva1, Tugba Boyunegmez Tumer2,1, Peter Kühn1, Allison J. Richard3, Shawna Wicks3, Jacqueline M. Stephens3, Zhong Wang3, Randy Mynatt3, William T. Cefalu3, Ilya Raskin1
1Department of Plant Biology and Pathology, Rutgers, The State University of New Jersey, New Brunswick, NJ, USA
2Department of Molecular Biology and Genetics Faculty of Arts and Sciences Çanakkale Onsekiz Mart University Çanakkale Turkey
3Pennington Biomedical Research Center, Baton Rouge, LA, USA

Tóm tắt

ScopeMoringa oleifera (moringa) is tropical plant traditionally used as an antidiabetic food. It produces structurally unique and chemically stable moringa isothiocyanates (MICs) that were evaluated for their therapeutic use in vivo.Methods and resultsC57BL/6L mice fed very high fat diet (VHFD) supplemented with 5% moringa concentrate (MC, delivering 66 mg/kg/d of MICs) accumulated fat mass, had improved glucose tolerance and insulin signaling, and did not develop fatty liver disease compared to VHFD‐fed mice. MC‐fed group also had reduced plasma insulin, leptin, resistin, cholesterol, IL‐1β, TNFα, and lower hepatic glucose‐6‐phosphatase (G6P) expression. In hepatoma cells, MC and MICs at low micromolar concentrations inhibited gluconeogenesis and G6P expression. MICs and MC effects on lipolysis in vitro and on thermogenic and lipolytic genes in adipose tissue in vivo argued these are not likely primary targets for the anti‐obesity and anti‐diabetic effects observed.ConclusionData suggest that MICs are the main anti‐obesity and anti‐diabetic bioactives of MC, and that they exert their effects by inhibiting rate‐limiting steps in liver gluconeogenesis resulting in direct or indirect increase in insulin signaling and sensitivity. These conclusions suggest that MC may be an effective dietary food for the prevention and treatment of obesity and type 2 diabetes.

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Tài liệu tham khảo

10.3389/fphar.2012.00024

10.1016/j.phytochem.2014.03.028

10.1016/j.bmc.2010.03.057

10.1002/mnfr.200800245

10.1016/j.bcp.2009.12.008

10.1021/np50111a011

Shapiro T. A., 2001, Chemoprotective glucosinolates and isothiocyanates of broccoli sprouts metabolism and excretion in humans, Cancer Epidem. Biomar., 10, 501

10.1016/j.phrs.2007.01.009

Verhoeven D. T., 1996, Epidemiological studies on brassica vegetables and cancer risk, Cancer Epidem. Biomar., 5, 733

10.1007/s11101-008-9103-7

10.1016/j.jff.2012.05.012

10.3109/09637486.2012.665043

10.1089/jmf.2012.2559

10.1016/j.carbpol.2010.05.020

10.1080/01635581.2011.589960

10.1038/485S14a

10.1016/S0140-6736(10)60550-8

Ng M., 2014, Global, regional, and national prevalence of overweight and obesity in children and adults during 1980–2013: a systematic analysis for the Global Burden of Disease Study 2013, Lancet, 6736, 60460

10.1016/S0021-9258(18)64849-5

10.1038/nprot.2008.73

10.1016/j.jnutbio.2010.09.004

10.1016/j.foodchem.2012.06.117

10.1016/0022-1759(83)90303-4

10.1371/journal.pone.0098897

10.3164/jcbn.40.229

10.1093/jn/138.6.1033

10.1152/ajpheart.00479.2008

10.1016/S1043-2760(00)00272-1

10.1038/39335

10.1172/JCI2849

10.1073/pnas.91.11.4854

10.1093/jn/138.9.1677

10.2337/diabetes.49.12.2063

10.1056/NEJMoa012512

10.1007/s00125-013-2991-0

10.2337/db13-0194

10.1038/nature13270

10.1210/jcem.82.2.3744

10.1038/35053000

10.1016/j.phrs.2005.05.004

10.1097/MOL.0b013e32834ae1a7

10.1021/jf9044827

10.1016/j.cmet.2012.11.006

10.1007/s00125-003-1244-z

10.1242/jcs.103.4.931

10.2337/db06-1687

10.1016/j.phrs.2006.03.009

10.1002/hep.24006

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Molecular Nutrition and Food Research

Tập 59 Số 6

1013-1024

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