Yu V. Kanevsky1, L. P. Pozdnyakova1, Yu V. Katukov1, С Е Северин1
1Moscow Research Institute of Medical Ecology, Simpferopolsky blvd. 8, Moscow, 113149, Russia
Tóm tắt
AbstractThe transferrin receptor (TFR) has been detected in tissues characterised by a high degree of proliferation. We have developed a procedure for isolating TFR from human placental tissues by affinity chromatography on transferrin‐Sepharose. Using gel filtration and electrophoresis in 7% PAAG, it has been shown that the molecular mass of the protein is 180 kDa. The protein has a subunit structure and is made up of two identical subunits, 90 kDa each. The constant for the protein binding to transferrin is equal to 5×10‐9 M. The yield of the protein isolated by the novel procedure exceeds 5‐fold that obtained by previously described methods.
