Isolation of nuclear shells from plant cells

Plant, Cell and Environment - Tập 11 Số 9 - Trang 819-825 - 1988
Zoya Galcheva-Gargova1, Emma M. Marinova2, Stanka Koleva2
1Institute of Molecular Biology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria
2Institute of Plant Physiology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria

Tóm tắt

Abstract. Nuclei from Zea mays and Phaseolus vulgaris root meristematic and differentiated cells were treated according to a recently developed simple procedure for isolation of nuclear lamina of Ehrlich Ascite Tumor (EAT) cells. As revealed by electron microscopy, the residual structures obtained represented empty nuclear shells, resembling those previously isolated from animal cells. Moreover, the composition of the residual nuclear structures from plant cells was found to be very similar to that described previously for the nuclear lamina purified by the adopted procedure. As demonstrated by SDS‐polyacrylamide gel electrophoresis, the plant nuclear shells contained a small number of proteins in the 65‐45 kD range. Two proteins — 62 and 50 kD—were most characteristic for beans, while a 55‐kD protein was abundant in maize. When blotted on nitrocellulose paper, some of the proteins of plant nuclear shells were immunoreactive with sera containing antibodies against the proteins of EAT nuclear envelopes. The degree of phosphorylation of the proteins of plant nuclear shells was found to be higher in meristematic than in differentiated maize root cells, correlating with the mitotic activity of the starting material.

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Thông tin xuất bản

Plant, Cell and Environment

Tập 11 Số 9

819-825

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