Isolation of Putative Progenitor Endothelial Cells for Angiogenesis

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NO release was measured with a NO-specific polarographic electrode connected to a NO meter (Iso-NO World Precision Instruments Sarasota FL). AT CD34+ or AT CD34− cells cultured in six-well plates were washed and then bathed in 5 ml of filtered Krebs-Henseleit solution. Cell plates were kept on a slide warmer (Lab Line Instruments Melrose Park IL) to maintain temperature between 35° and 37°C. The sensor probe was inserted vertically into the wells and the tip of the electrode was positioned 2 mm under the surface of the solution.

Supported by grants from NIH National Heart Lung and Blood Institute numbers 02824 53354 and 57516 the American Heart Association the E. L. Wiegand Foundation and in part by the Uehara Memorial Foundation (T.M.).