Isolation of Bacteriophages Specific to a Fish Pathogen, Pseudomonas plecoglossicida , as a Candidate for Disease Control

Applied and Environmental Microbiology - Tập 66 Số 4 - Trang 1416-1422 - 2000
Se Chang Park1, Ichiro Shimamura2, Minoru Fukunaga2, Koh-ichiro Mori3, Toshihiro Nakai1
1Fish Pathology Laboratory, Faculty of Applied Biological Science, Hiroshima University, Higashi-hiroshima, Hiroshima,1
2Tokushima Prefectural Fisheries Experimental Station, Hiwasa, Tokushima,2 and
3Kamiura Station, Japan Sea-Farming Association, Kamiura, Oita,3Japan

Tóm tắt

ABSTRACT Two types of bacteriophage specific to Pseudomonas plecoglossicida , the causative agent of bacterial hemorrhagic ascites disease in cultured ayu fish ( Plecoglossus altivelis ), were isolated from diseased ayu and the rearing pond water. One type of phage, which formed small plaques, was tentatively classified as a member of the family Myoviridae , and the other type, which formed large plaques, was classified as a member of the family Podoviridae . All 27 strains of P. plecoglossicida examined, which were isolated from diseased ayu from geographically different areas in 1991 to 1999, exhibited quite similar sensitivities to either type of phage. One strain of P. plecoglossicida was highly virulent for ayu, and the 50% lethal dose (LD 50 ) when intramuscular injection was used was 10 1.2 CFU fish −1 ; in contrast, phage-resistant variants of this organism were less virulent (LD 50 , >10 4 CFU fish −1 ). Oral administration of phage-impregnated feed to ayu resulted in protection against experimental infection with P. plecoglossicida . After oral administration of P. plecoglossicida cells of this bacterium were always detected in the kidneys of control fish that did not receive the phage treatment, while the cells quickly disappeared from the phage-treated fish. Bacterial growth in freshwater was lower in the presence of phage, and the number of phage PFU increased rapidly. These results suggest that it may be possible to use phage to control the disease caused by P. plecoglossicida .

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Applied and Environmental Microbiology

Tập 66 Số 4

1416-1422

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