Isolation, characterization, and expression of cDNA clones that encode rat UDP‐galactose: Ceramide galactosyltransferase

Journal of Neuroscience Research - Tập 38 Số 2 - Trang 234-242 - 1994
Norbert Stahl1,2, Helga Jurevics1, Pierre Morell1,2, Kotaro Suzuki1,3, Brian Popko1,2,4
1Brain and Development Research Center, University of North Carolina at Chapel Hill
2Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill
3Department of Neurology and Psychiatry, University of North Carolina at Chapel Hill
4Program in Molecular Biology and Biotechnology, University of North Carolina at Chapel Hill

Tóm tắt

AbstractUDP‐galactose:ceramide galactosyltransferase (CGT) (EC. 2.4.1.62) catalyzes the final step in the synthesis of galactocerebroside (GalC), a glycosphingolipid found in high amounts in the myelin sheath. Here, the isolation of rat CGT specific cDNA clones is reported. The CGT sequence contains an open reading frame of 1,623 bp which predicts a protein of Mr 61,126 Da. In transfection experiments the cDNA was found to confer CGT activity of Chinese hamster ovary cells. In rat brain the developmental expression pattern of CGT mRNA was similar to the myelination profile, whereas the sciatic nerve contained high amounts of CGT message over a long developmental period. CGT mRNA expression in the sciatic nerve was found to drop substantially following nerve injury and recover slowly when compared to the expression of mRNAs specific for the predominant myelin‐specific proteins. The absolute amounts of CGT message in sciatic nerve and brain were found to be comparable to those that encode the structural proteins of myelin. Except for low amounts in the kidney, the CGT mRNA was not detected in other tissues examined. Southern blot analysis revealed that the CGT protein is likely encoded by a single, relatively large gene. © 1994 Wiley‐Liss, Inc.

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Thông tin xuất bản

Journal of Neuroscience Research

Tập 38 Số 2

234-242

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