Isolation, characterisation and identification of a potyvirus from Dioscorea alata L. (water yam) in Nigeria

Annals of Applied Biology - Tập 134 Số 1 - Trang 65-71 - 1999
B. O. Odu1, J. Hughes2, S. A. Shoyinka3, L. N. Dongo2
1Department of Botany and Microbiology, Faculty of Science, University oflbadan, Nigeria
2International Institute of Tropical Agriculture, Oyo Road, PMB 5320, Ibadan, Nigeria
3Institute of Agricultural Research and Training, Moor Plantation, P.M.B. 5029, Ibadan, Nigeria

Tóm tắt

Summary

A yam potyvirus was isolated from Dioscorea alata samples collected in Nigeria. The virus was not transmissible mechanically but was transmitted by Aphis craccivora to four cowpea cultivars (Ife Brown, IT84S‐2114, IT82E‐10 and TVu2657), and from which it could be mechanically transmitted between the cowpea cultivars. In infectivity‐ tests using cowpea extracts, the virus had a dilution end point of 10‐4, a thermal inactivation point of 60–65°C and longevity in vitro of 2 days at room temperature. The virus coat protein had an estimated molecular weight of 32 100 daltons. The virus was identified as an isolate of Dioscorea alata virus (DAV; syn. yam virus 1) due to its biological characteristics and its serological reaction with antiserum raised against DAV. The virus is not related to yam mosaic virus, but distantly related to blackeye cowpea mosaic virus and cowpea aphid‐borne mosaic virus.

Từ khóa


Tài liệu tham khảo

Anderson C. W., 1955, Vigna and Crotalaria viruses in Florida. 2: Notations concerning cowpea mosaic virus (Marmor vignae), Plant Disease Reporter, 39, 349

Anon., 1995, Annual Report of IITA for 1971–1993, 38

10.1111/j.1744-7348.1973.tb07724.x

Chant S. R., 1957, Annual Report

Coursey D. G., 1967, Yams, 230

Deighton F. C., 1956, Diseases of cultivated and other economic plants in Sierra Leone, 77

10.1016/0166-0934(85)90024-2

Fauquet C., 1987, Plant viral diseases in the Ivory Coast

HughesJ d'A.1986.Viruses of theAraceaeandDioscoreaspecies: Their isolation characterization and detection.Ph.D. Thesis University of Reading UK.

10.1111/j.1744-7348.1988.tb02046.x

Hughes J d'A, 1997, Viruses infecting cultivated yams (Dioscorea alata and D. rotundata) in Nigeria, Phytopathology, 87

10.1111/j.1462-5822.2007.00901.x

Lawani S. M., 1976, A bibliography of Yams and the genus, 192

10.1007/BF02650216

10.1094/PD-64-925

MartinF. W.1976.Selected yam varieties for the tropics. InProceeding of the 4th Symposium of the International Society for Tropical Root Crops pp.44–49. Held at CIAT Cali Colombia 1–7 August 1976. Eds.J.Cock R.MaclntyreandM.Graham.

10.1111/j.1439-0434.1987.tb04430.x

RobertsonD. G.1961.Notes in Annual Reports. Federal Department of Agriculture Research Nigeria. 1959–1960..

RosselH. W. ThottappillyG.1985.Virus diseases of important food crops in Tropical Africa.IITA Publication Series.International Institute of Tropical Agriculture Ibadan.61pp.

Taiwo M. A., 1988, Virus diseases of plant in Africa, 93

Terry E. R., 1976, Proceedings of the 4th Symposium of the International Society for Tropical Root Crops, 170

10.1111/j.1744-7348.1979.tb06542.x

Thông tin
Thông tin xuất bản

Annals of Applied Biology

Tập 134 Số 1

65-71

Thông tin tác giả