Isolation and characterization of microsatellite loci in the striped mullet, Mugil cephalus

Wiley - Tập 5 Số 2 - Trang 323-326 - 2005
Emanuela Miggiano1, Russell E. Lyons2, Yutao Li2, Leanne Dierens2, D. Crosetti3, Luís Gil de Sola1
1Department of Animal and Human Biology, University of Rome La Sapienza, Via. Borelli, 50, 00161 Rome, Italy.
2CSIRO Livestock Industries, 306 Carmody Road, St. Lucia 4067, Queensland, Australia
3ICRAM, Central Institute for Marine Research, Via di Casalotti, 300, 00166 Rome, Italy,

Tóm tắt

AbstractForty‐three microsatellites were isolated from grey mullet (Mugil cephalus) of Mediterranean origin using the standard enrichment methods followed by enhanced chemiluminescence for the rapid identification of clones containing microsatellites. Eleven microsatellites were characterized to assess genetic diversity of different populations surrounding the island of Sardinia in Italy. The versatility of these markers was also assessed using an Australian population. All 11 microsatellites were highly polymorphic with a mean heterozygosity of 0.834. The number of alleles ranged from 14 to 38 in the Sardinian populations and from 11 to 25 in the Australian samples.

Từ khóa


Tài liệu tham khảo

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Thông tin xuất bản

Wiley

Tập 5 Số 2

323-326

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