Nội dung được dịch bởi AI, chỉ mang tính chất tham khảo
Cách ly và đặc trưng tế bào ngà tủy từ đầu gốc của răng hàm số ba của người và sự biệt hóa của chúng thành tế bào xi-măng: một nghiên cứu in vitro
Tóm tắt
Sự tái sinh nha chu, điều trị các bệnh liên quan đến nha chu và cải thiện chức năng của các cấy ghép là những thách thức điều trị toàn cầu. Sự biệt hóa tế bào gốc người từ ngà tủy thành các tế bào xi măng có thể cung cấp một chiến lược cho việc điều trị viêm nha chu. Nghiên cứu này nhằm đánh giá quá trình biệt hóa của tế bào gốc người nguyên phát ngà tủy (hSCAPs) sang các tế bào xi măng. Các tế bào SCAPs được tách ra từ răng hàm số ba của người và được ủ trong 21 ngày trong một môi trường vi mô biệt hóa. Các thử nghiệm nhuộm phosphatase kiềm (ALP) và Alizarin đỏ S được thực hiện để đánh giá sự lắng đọng canxi và hình thành hydroxyapatite trong môi trường nuôi cấy hSCAPs. Thử nghiệm phản ứng chuỗi polymerase thời gian thực (RT-PCR) đã được thực hiện cho protein xi măng 1 (CEMP1), collagen loại I (COL1), F-spondin (SPON1), osteocalcin (OCN), và osteopontin (OPN) như là các dấu hiệu đặc trưng của tế bào xi măng và các tiền thân của chúng. Hoạt động phosphatase ALP vào ngày 21 của quá trình điều trị cho thấy sự gia tăng có ý nghĩa về ALP so với mẫu kiểm soát. Thử nghiệm nhuộm Alizarin đỏ S cho thấy rằng các hSCAPs đã biệt hóa cung cấp một số lượng lớn các nốt lắng đọng canxi so với mẫu kiểm soát. Mức độ biểu hiện tăng cao của CEMP1, OCN, OPN, COL1 và Spon1 được quan sát thấy vào ngày 7, 14 và 21 so với mẫu kiểm soát, trong khi mức độ biểu hiện cao nhất được quan sát vào ngày 21. Kết luận, môi trường vi mô biệt hóa là tiện lợi và hữu ích để thúc đẩy sự biệt hóa của hSCAPs thành tế bào xi măng.
Từ khóa
#tế bào gốc người #ngà tủy #tế bào xi măng #viêm nha chu #biệt hóa tế bàoTài liệu tham khảo
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