Isolation and characterization of a tumor necrosis factor binding protein from urine

European Journal of Haematology - Tập 42 Số 3 - Trang 270-275 - 1989
Inge Olsson1, Mikael Lantz2, Eva Nilsson2, Christina Peetre2, Hans Thysell3, Anders Grubb4, G.R. Adolf5
1Department of Medicine, Lund hospital, Sweden.
2Division of Hematology, Department of Medicine, Lund
3Department of Nephrology, Lund Hospital, Lund
4Department of Clinical Chemistry, Malmö, Sweden
5Ernst Boehringer Institut für Arzneimittelforschung, Vienna, Austria

Tóm tắt

Tumor necrosis factor (TNF)/cachectin can produce both beneficial and harmful manifestations. Mechanisms may operate to counteract potentially harmful effects such as shock and cachexia. The TNF binding protein (TNF‐BP), which is found at increased levels in serum and urine of patients with chronic renal failure, may play such a role. TNF‐BP was purified 1000000–fold to homogeneity from urine of patients with chronic renal failure by use of ion exchange chromatography, affinity chromatography on TNF‐Sepharose and reverese phase chromatography. The purified protein contained only one chain with an apparent Mr on sodium dodecyl sulfate‐polyacrylamide gel electrophoresis of 30000. The aminoterminal amino acid sequence D‐S‐V‐X‐P‐Q‐G‐K‐Y‐I‐H‐P‐Q‐V‐N‐S‐I‐X‐K‐T revealed no significant homologies with previously described protein sequences. TNF‐BP may act as a regulator of the bioactivities of TNF/cachectin.

Từ khóa


Tài liệu tham khảo

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Thông tin
Thông tin xuất bản

European Journal of Haematology

Tập 42 Số 3

270-275

Thông tin tác giả