Isolation and Characterization of Tumorigenic, Stem-like Neural Precursors from Human Glioblastoma

Cancer Research - Tập 64 Số 19 - Trang 7011-7021 - 2004
Rossella Galli1, Elena Binda1, Ugo Orfanelli1, Barbara Cipelletti1, Angela Gritti1, Simona De Vitis2, Roberta Fiocco1, Chiara Foroni1, Francesco DiMeco3,4, Angelo L. Vescovi1,5
11Stem Cell Research Institute and
22Laboratory of Molecular Diagnostics, H. S. Raffaele, Milan, Italy;
34Department of Neurological Surgery, Johns Hopkins Medical School, Baltimore, Maryland; and
4Department of Neurological Surgery, Johns Hopkins Medical School, Baltimore, Maryland
55Department of Biological Sciences and Biotechnology, University of Milano-Bicocca, Milan, Italy

Tóm tắt

Abstract Transformed stem cells have been isolated from some human cancers. We report that, unlike other brain cancers, the lethal glioblastoma multiforme contains neural precursors endowed with all of the critical features expected from neural stem cells. Similar, yet not identical, to their normal neural stem cell counterpart, these precursors emerge as unipotent (astroglial) in vivo and multipotent (neuronal-astroglial-oligodendroglial) in culture. More importantly, these cells can act as tumor-founding cells down to the clonal level and can establish tumors that closely resemble the main histologic, cytologic, and architectural features of the human disease, even when challenged through serial transplantation. Thus, cells possessing all of the characteristics expected from tumor neural stem cells seem to be involved in the growth and recurrence of adult human glioblastomas multiforme.

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