Irisin promotes odontogenic differentiation and angiogenic potential in human dental pulp cells
Tóm tắt
To determine whether irisin, a newly discovered myokine that links exercise‐induced and metabolic homeostasis, is able to promote odontogenic differentiation and angiogenesis in human dental pulp cells (HDPCs).
Cell viability in the presence of irisin was measured. Real‐time PCR and Western blot analysis were performed to evaluate the expression levels of irisin, odontogenic and angiogenic markers. The involvement of mitogen‐activated protein kinase (MAPK) and the protein kinase B (Akt) signalling pathway was evaluated by Western blot. To evaluate mineralization nodule formation, alkaline phosphatase (ALP) staining and alizarin red S staining were performed. Scratch wound assays were performed to evaluate the effects of irisin on cell migration. The data were analysed using one‐way analysis of variance (
Irisin significantly promoted odontogenic differentiation as evidenced by formation of mineralized nodules, induction of ALP activity and upregulation of odontogenic and angiogenic markers (
Irisin promoted odontogenic differentiation and mineralization and has the potential for angiogenesis through activation of the MAPK and Akt signalling pathways in HDPCs.
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Tài liệu tham khảo
Elkasrawy MN, 2010, Myostatin (GDF‐8) as a key factor linking muscle mass and skeletal form, Journal of Musculoskeletal & Neuronal Interactions, 10, 56
ISO 10993–5, 2009, Biological evaluation of medical devices. Part 5: Tests for cytotoxicity: in vitro methods