Irisin promotes odontogenic differentiation and angiogenic potential in human dental pulp cells

International Endodontic Journal - Tập 54 Số 3 - Trang 399-412 - 2021
Ji-won Son1, Sung-Hyeon Choi1, Ji‐Hyun Jang2, Jeong‐Tae Koh3, Won‐Mann Oh1, Yun‐Chan Hwang1, Bin-Na Lee1
1Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, Korea
2Department of Conservative Dentistry, School of Dentistry, Kyung Hee University, Seoul, Korea
3Department of Pharmacology and Dental Therapeutics, Hard-tissue Biointerface Research Center, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, Korea

Tóm tắt

AbstractAimTo determine whether irisin, a newly discovered myokine that links exercise‐induced and metabolic homeostasis, is able to promote odontogenic differentiation and angiogenesis in human dental pulp cells (HDPCs).MethodologyCell viability in the presence of irisin was measured. Real‐time PCR and Western blot analysis were performed to evaluate the expression levels of irisin, odontogenic and angiogenic markers. The involvement of mitogen‐activated protein kinase (MAPK) and the protein kinase B (Akt) signalling pathway was evaluated by Western blot. To evaluate mineralization nodule formation, alkaline phosphatase (ALP) staining and alizarin red S staining were performed. Scratch wound assays were performed to evaluate the effects of irisin on cell migration. The data were analysed using one‐way analysis of variance (anova) followed by Tukey post hoc test and Student’s t‐test. Statistical significance was considered at P < 0.05.ResultsIrisin significantly promoted odontogenic differentiation as evidenced by formation of mineralized nodules, induction of ALP activity and upregulation of odontogenic and angiogenic markers (P < 0.05). Scratch wound assays revealed that irisin significantly increased migration of HDPCs (P < 0.05). Phosphorylation of both MAPK and Akt was increased by irisin. MAPK and Akt inhibitors inhibited mineralization, cell migration and the increased expression of odontogenic and angiogenic markers.ConclusionsIrisin promoted odontogenic differentiation and mineralization and has the potential for angiogenesis through activation of the MAPK and Akt signalling pathways in HDPCs.

Từ khóa


Tài liệu tham khảo

10.1038/nrc1753

10.1016/j.bone.2010.12.008

10.1097/00004770-200009000-00010

10.1038/nature10777

10.1016/S0968-0004(97)01074-8

10.1111/j.1752-7325.2005.tb02792.x

10.1038/74651

10.1002/jcp.21234

10.1073/pnas.1516622112

10.1038/s41598-017-02557-8

10.1111/iej.12489

10.1016/S0165-6147(00)01676-X

10.1038/nrd3686

Elkasrawy MN, 2010, Myostatin (GDF‐8) as a key factor linking muscle mass and skeletal form, Journal of Musculoskeletal & Neuronal Interactions, 10, 56

10.1007/3-7643-7311-3_15

10.1159/000324254

10.1074/jbc.273.46.30336

10.1177/0022034509340867

ISO 10993–5, 2009, Biological evaluation of medical devices. Part 5: Tests for cytotoxicity: in vitro methods

10.1016/j.mrrev.2012.08.001

10.1016/j.msec.2014.06.025

10.1006/meth.2001.1262

10.1016/j.archoralbio.2016.08.011

10.1177/00220345000790081201

10.1016/j.joen.2009.11.021

10.1177/154405910808701204

10.1177/0022034511405323

10.1016/j.joen.2017.11.018

10.1007/s12015-016-9661-9

10.1016/S8756-3282(01)00683-4

10.1038/nrendo.2012.49

10.1038/nrendo.2016.221

10.1038/srep18732

10.1038/386671a0

10.1016/S0003-9969(00)00075-3

10.1155/2016/5957806

10.1371/journal.pone.0110273

10.1177/0022034511408426

10.1111/odi.12227

10.1002/term.1899

10.1016/j.archoralbio.2007.07.001

10.1155/2019/4793518

10.1371/journal.pone.0134662

10.2147/OTT.S214260

10.1038/boneres.2016.56

Thông tin
Thông tin xuất bản

International Endodontic Journal

Tập 54 Số 3

399-412

Thông tin tác giả