Investigation of structural stability and functionality of homodimeric gramicidin towards peptide‐based drug: a molecular simulation approach

Journal of Cellular Biochemistry - Tập 120 Số 4 - Trang 4903-4911 - 2019
P. Ganesan1, R. Rajasekaran1
1Bioinformatics Lab, Department of Biotechnology, School of Biosciences and Technology, Vellore Institute of Technology (VIT), Vellore, India

Tóm tắt

AbstractIncreasing death rates due to antibiotic resistance deteriorate the existing treatment measures. Antimicrobial peptides have turned into the emerging cure for multidrug resistance. However, the stability and functionality determine an antimicrobial peptide as a drug. Analyses of the homodimeric β‐helical peptide, gramicidin have suggested the significant role of gramicidin‐A, gramicidin‐B, and gramicidin‐C as antimicrobial compounds, but the structural basis for understanding the stability and functionality is insufficient to resolve multidrug resistance. To identify the best template among gramicidin types as a therapeutic product, we combined a detailed comparative static analysis and dynamic analysis along with conformational free energy and secondary structure prediction. We observed that the high intramolecular interactions and the geometrical features favored gramicidin‐A among other types of gramicidin. Our analyses further revealed that the secondary structure of gramicidin‐A showed β sheets with coils along the conformations without any disruption, thereby enhanced its membrane interactions in terms of binding free energy. In conclusion, gramicidin‐A has definitely showed enhanced structural stability and functionality; this could be considered the best template for a potential therapeutic product.

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Journal of Cellular Biochemistry

Tập 120 Số 4

4903-4911

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