Intracellular killing of mastitis pathogens by penethamate hydriodide following internalization into mammary epithelial cells

Journal of Veterinary Pharmacology and Therapeutics - Tập 30 Số 2 - Trang 151-156 - 2007
R.A. Almeida1, D. Patel1, Gabriele M Friton2, S.P. Oliver1
1Department of Animal Science & Food Safety Center of Excellence, The University of Tennessee, Knoxville, TN, USA
2Boehringer Ingelheim Animal Health GmbH, Ingelheim/Rhein, Germany.

Tóm tắt

Penethamate hydriodide was highly effective in killing Streptococcus uberis, Streptococcus dysgalactiae subsp. dysgalactiae and Staphylococcus aureus that internalized mammary epithelial cells. At higher concentrations (32 μg/mL to 32 mg/mL), killing rates ranged from 85% to 100%. At lower concentrations (0.032 μg/mL to 3.2 μg/mL), killing rates ranged from 0 to 80%. Results of this proof‐of‐concept study demonstrated that: (1) penethamate hydriodide is capable of entering mammary epithelial cells and killing intracellular mastitis pathogens without affecting mammary epithelial cell viability, (2) the in vitro model used is capable of quantifying the fate of mastitis pathogens internalized into mammary epithelial cells, and (3) this in vitro model can be used to determine the effectiveness of antibiotics at killing bacteria within the cytoplasm of mammary epithelial cells.

Từ khóa


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Thông tin
Thông tin xuất bản

Journal of Veterinary Pharmacology and Therapeutics

Tập 30 Số 2

151-156

Thông tin tác giả