Interaction of Porin from Yersinia pseudotuberculosis with Different Structural Forms of Endogenous Lipopolysaccharide

Biochemistry (Moscow) - Tập 68 - Trang 976-983 - 2003
T. I. Vakorina1, O. D. Novikova1, I. N. Krasikova1, G. N. Naberezhnykh1, T. F. Solov'eva1, Yu. S. Ovodov2
1Far East Branch of the Russian Academy of Sciences, pr. 100-letiya Vladivostoka 159, Pacific Institute of Bioorganic Chemistry, Vladivostok, Russia
2Urals Branch of the Russian Academy of Sciences, ul. Pervomaiskaya 50, Institute of Physiology, Komi Science Center, Syktyvkar, Russia

Tóm tắt

The interaction of Yersinia pseudotuberculosis porin solubilized in deoxycholate with the S- and R-forms of endogenous lipopolysaccharide (LPS) was studied by the quenching of intrinsic protein fluorescence. The samples of S-LPS differed both in the length of O-specific polysaccharide (n = 1 and 4) and in the acylation degree of the 3-hydroxytetradecanoic acid residues of the lipid A moiety (12-66%). R-LPS (12%) binding to porin was found to occur with positive cooperativity on two integrated structural regions of the R-LPS macromolecule, namely, core oligosaccharide and lipid A. The mode of porin interaction with low-acylated S-LPSs (15 or 20%) coincided with a model involving three types of binding sites. The shape of Scatchard curves of binding indicates that a complex formation between porin and low-acylated S-LPS is cooperative at low and moderate ligand concentration, whereas at near-saturating LPS concentrations porin binds to LPS independently on two types of binding sites. The O-specific polysaccharide chain in the S-LPS macromolecule increases the affinity of its interaction with porin in comparison with R-LPS–porin binding. A significant increase (to 66%) in the degree of S-LPS acylation substantially changed its porin-binding character: the process becomes anti-cooperative with lowered affinity. Thus, the features of LPS–porin interaction significantly depend on the conformational changes in the LPS molecule due to expanding of its hydrophobic region.

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Biochemistry (Moscow)

Tập 68

976-983

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