Inhibition ofNO2,PGE2, TNF-α,andiNOS EXpression byShorea robustaL.: An Ethnomedicine Used for Anti-Inflammatory and Analgesic Activity

Evidence-based Complementary and Alternative Medicine - Tập 2012 - Trang 1-14 - 2012
Chattopadhyay Debprasad1, Hemanta Mukherjee1, Paromita Bag1, Durbadal Ojha1, Konreddy Ananda Kumar2, Shanta Dutta3, Haldar Pallab Kumar4, Tirthankar Chatterjee4, Ashoke Sharon2, Sekhar Chakraborti3,1
1ICMR Virus Unit, ID and BG Hospital, GB 4, First Floor, 57 Dr. Suresh C Banerjee Road, Beliaghata, Kolkata 700010, India
2Department of Applied Chemistry, Birla Institute of Technology, Mesra, Ranchi 835215, India
3Division of Microbiology, National Institute of Cholera and Enteric Diseases, Kolkata 700010, India
4Division of Pharmacology, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, India

Tóm tắt

This paper is an attempt to evaluate the anti-inflammatory and analgesic activities and the possible mechanism of action of tender leaf extracts ofShorea robusta, traditionally used in ailments related to inflammation. The acetic-acid-induced writhing and tail flick tests were carried out for analgesic activity, while the anti-inflammatory activity was evaluated in carrageenan-and dextran- induced paw edema and cotton-pellet-induced granuloma model. The acetic-acid-induced vascular permeability, erythrocyte membrane stabilization, release of proinflammatory mediators (nitric oxide and prostaglandin E2), and cytokines (tumor necrosis factor-α, and interleukins-1β and -6) from lipopolysaccharide-stimulated human monocytic cell lines were assessed to understand the mechanism of action. The results revealed that both aqueous and methanol extract (400 mg/kg) caused significant reduction of writhing and tail flick, paw edema, granuloma tissue formation (P<0.01), vascular permeability, and membrane stabilization. Interestingly, the aqueous extract at 40 μg/mL significantly inhibited the production of NO and release of PGE2, TNF-α, IL-1β, and IL-6. Chemically the extract contains flavonoids and triterpenes and toxicity study showed that the extract is safe. Thus, our study validated the scientific rationale of ethnomedicinal use ofS. robustaand unveils its mechanism of action. However, chronic toxicological studies with active constituents are needed before its use.

Từ khóa


Tài liệu tham khảo

1985, Survey and synthesis of pathology research, 4, 44

1993, Journal of Experimental Medicine, 178, 63, 10.1084/jem.178.1.63

10.1111/j.1365-2249.2006.03261.x

10.1016/j.archger.2003.10.001

10.1016/S0378-8741(02)00379-3

10.1016/S1387-2656(08)00012-4

1993, 1

1986, Journal of Ethnopharmacology, 15, 57, 10.1016/0378-8741(86)90104-2

10.1055/s-2001-18857

2008, Pharmacologyonline, 1, 9

1998

1989

10.1016/S0378-8741(01)00264-1

2005, Oriental Pharmacy and Experimental Medicine, 5, 124, 10.3742/OPEM.2005.5.2.124

2009, Journal of Pharmacognosy and Phytotherapy, 1, 1

1996

1997

1959, Federation Proceedings, 18, 412

2003, Drug Discovery Today, 8

1962, Experimental Biology and Medicine, 111, 544, 10.3181/00379727-111-27849

10.1016/S0378-8741(02)00165-4

2002, Phytomedicine, 9, 632, 10.1078/094471102321616445

1996, Indian Journal of Pharmaceutical Sciences, 58, 67

1960, Journal of Pharmacy and Pharmacology, 12, 659, 10.1111/j.2042-7158.1960.tb12727.x

1964, British Journal of Pharmacology, 22, 246

10.1016/S0367-326X(99)00030-1

10.1373/clinchem.2005.056093

10.1248/bpb.28.1668

1984

2001, Pharmazie, 56, 465

1993, Phytochemistry, 32, 466, 10.1016/S0031-9422(00)95019-2

10.1016/S0031-9422(97)00004-6

10.1039/a804621b

10.1016/S0367-326X(99)00075-1

2002, Indian Journal of Chemistry Section B, 41, 1097

10.2165/00003495-199855040-00001

10.1002/ptr.942

1973, Nature, 246, 215, 10.1038/246215a0

1999, Journal of Allergy and Clinical Immunology, 103, S378, 10.1016/S0091-6749(99)70215-0

1989, Agents and Actions, 28, 78, 10.1007/BF02022984

10.1074/jbc.274.12.7611

1995, American Journal of Clinical Pathology, 103, 466, 10.1093/ajcp/103.4.466

10.1182/blood.V98.4.1231

2006, Histamine, bradykinin, and their antagonists, 645

1996, Inflammation, healing and repair, 112

1995, International Journal of Pharmacognosy, 33, 65, 10.3109/13880209509088150

1988, The FASEB Journal, 2, 2867, 10.1096/fasebj.2.13.2844616

1992, Biochemical Journal, 285, 295, 10.1042/bj2850295

1995, Drugs, 49, 345, 10.2165/00003495-199549030-00003

10.1016/0378-8741(95)01257-E

1996, Biochemical Pharmacology, 51, 737, 10.1016/0006-2952(95)02172-8

1999, Natural Medicines, 53, 113

10.1073/pnas.91.25.12013

1994, Proceedings of the National Academy of Sciences of the United States of America, 91, 3228, 10.1073/pnas.91.8.3228

1994, Journal of Leukocyte Biology, 56, 559, 10.1002/jlb.56.5.559

10.1146/annurev.immunol.14.1.397

10.1016/0304-3959(95)00186-7

10.2174/1566524043360636

1996, Veterinary Pathology, 33, 662, 10.1177/030098589603300605

1990, Journal of Immunology, 145, 2932, 10.4049/jimmunol.145.9.2932

1996, Journal of Leukocyte Biology, 59, 598, 10.1002/jlb.59.4.598

10.1016/j.cytogfr.2005.03.005

10.1073/pnas.95.15.8841

10.1016/S0142-9612(00)00078-8

2002, The Spine Journal, 2

2000, Pharmacological Reviews, 52, 673

10.1016/S0163-7258(02)00298-X

10.1159/000092859

Thông tin
Thông tin xuất bản

Evidence-based Complementary and Alternative Medicine

Tập 2012

1-14

Thông tin tác giả