Inhibition of NF-kappaB transcriptional activity enhances fucoxanthinol-induced apoptosis in colorectal cancer cells
Tóm tắt
Evidence from epidemiological and experimental studies has shown that the etiology of colorectal cancer (CRC) is related to lifestyle, mainly diet. At the same time, there are many foods and beverages that have been shown to provide protection against CRC. We turned our attention to a traditional Japanese food, brown algae, that contains carotenoids and various functional polyphenols, especially fucoxanthin (FX) and fucoxanthinol (FxOH). Both FX and FxOH treatments induced apoptosis in a dose-dependent and time-dependent manner as detected by annexin V / propidium iodide and the presence of a subG1 population in human colon cancer HCT116 cells. This apoptotic effect of FxOH was stronger than that of FX. We also found that nuclear factor-kappa B (NF-κB) transcriptional activity was significantly increased by treatment with ≥5 μM FxOH. Thus, we cotreated the cells with FxOH plus NF-κB inhibitor, and the results demonstrated that this cotreatment strongly enhanced the induction of apoptosis compared with the effects of FxOH or NF-κB inhibitor treatment alone and resulted in X-linked inhibitor of apoptosis (IAP) downregulation. This study suggested that FxOH is a more potent apoptosis-inducing agent than FX and that its induction of apoptosis is enhanced by inhibiting NF-κB transcriptional activity via suppression of IAP family genes.
Tài liệu tham khảo
American Cancer Society. Cancer Facts and Figures 2015. Atlanta: American CancerSociety2015; Available from:
https://www.cancerorg/content/dam/cancer-org/research/cancer-facts-and-statistics/annual-cancer-facts-and-figures/2015/cancer-facts-and-figures-2015.pdf
.
citation_journal_title=Gut; citation_title=Global patterns and trends in colorectal cancer incidence and mortality; citation_author=M Arnold, MS Sierra, M Laversanne, I Soerjomataram, A Jemal, F Bray; citation_volume=66; citation_publication_date=2017; citation_pages=683-691; citation_doi=10.1136/gutjnl-2015-310912; citation_id=CR2
Cancer Statistics in Japan '17. Center for Cancer Control and Information Services, National Cancer Center. Available from:
https://ganjoho.jp/en/professional/statistics/brochure/2017_en.html
citation_journal_title=Ann Oncol; citation_title=Foods and beverages and colorectal cancer risk: a systematic review and meta-analysis of cohort studies, an update of the evidence of the WCRF-AICR continuous update project; citation_author=AR Vieira, L Abar, DSM Chan, S Vingeliene, E Polemiti, C Stevens, D Greenwood, T Norat; citation_volume=28; citation_publication_date=2017; citation_pages=1788-1802; citation_doi=10.1093/annonc/mdx171; citation_id=CR4
citation_journal_title=World J Pharmacol; citation_title=Potential ability of xanthophylls to prevent obesity-associated cancer; citation_author=M Terasaki, M Mutoh, G Fujii, M Takahashi, R Ishigamori, S Masuda; citation_volume=3; citation_publication_date=2014; citation_pages=140-152; citation_doi=10.5497/wjp.v3.i4.140; citation_id=CR5
citation_journal_title=J Oleo Sci; citation_title=Effect of medium-chain triacylglycerols on anti-obesity effect of fucoxanthin; citation_author=H Maeda, M Hosokawa, T Sashima, K Funayama, K Miyashita; citation_volume=56; citation_publication_date=2007; citation_pages=615-621; citation_doi=10.5650/jos.56.615; citation_id=CR6
citation_journal_title=Diabetes Obes Metab; citation_title=The effects of Xanthigen in the weight management of obese premenopausal women with non-alcoholic fatty liver disease and normal liver fat; citation_author=M Abidov, Z Ramazanov, R Seifulla, S Grachev; citation_volume=12; citation_publication_date=2010; citation_pages=72-81; citation_doi=10.1111/j.1463-1326.2009.01132.x; citation_id=CR7
citation_journal_title=Oncol Rep; citation_title=Glycine and succinic acid are effective indicators of the suppression of epithelial-mesenchymal transition by fucoxanthinol in colorectal cancer stem-like cells; citation_author=M Terasaki, M Mima, S Kudoh, T Endo, H Maeda, J Hamada, K Osada, K Miyashita, M Mutoh; citation_volume=40; citation_publication_date=2018; citation_pages=414-424; citation_id=CR8
citation_journal_title=Anticancer Res; citation_title=Glycine is a predictor for a suppressive effect of fucoxanthinol on colonosphere formation under hypoxia; citation_author=M Terasaki, Y Ogawa, T Endo, H Maeda, J Hamada, K Osada, K Miyashita, M Mutoh; citation_volume=38; citation_publication_date=2018; citation_pages=2169-2179; citation_id=CR9
citation_journal_title=Nutr Cancer; citation_title=Induction of anoikis in human colorectal cancer cells by fucoxanthinol; citation_author=M Terasaki, H Maeda, K Miyashita, M Mutoh; citation_volume=69; citation_publication_date=2017; citation_pages=1043-1052; citation_doi=10.1080/01635581.2017.1339814; citation_id=CR10
citation_journal_title=Cytometry; citation_title=Features of apoptotic cells measured by flow cytometry; citation_author=Z Darzynkiewicz, S Bruno, G Bino, W Gorczyca, MA Hotz, P Lassota, F Traganos; citation_volume=13; citation_publication_date=1992; citation_pages=795-808; citation_doi=10.1002/cyto.990130802; citation_id=CR11
citation_journal_title=J Immunol Methods; citation_title=A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry; citation_author=I Nicoletti, G Migliorati, MC Pagliacci, F Grignani, C Riccardi; citation_volume=139; citation_publication_date=1991; citation_pages=271-279; citation_doi=10.1016/0022-1759(91)90198-O; citation_id=CR12
citation_journal_title=J Clin Immunol; citation_title=The anti-death machinery in IKK/NF-kappaB signaling; citation_author=JL Luo, H Kamata, M Karin; citation_volume=25; citation_publication_date=2005; citation_pages=541-550; citation_doi=10.1007/s10875-005-8217-6; citation_id=CR13
citation_journal_title=Nat Med; citation_title=Control of inducible chemoresistance: enhanced anti-tumor therapy through increased apoptosis by inhibition of NF-κB; citation_author=CY Wang, JC Cusack, R Liu, AS Baldwin; citation_volume=5; citation_publication_date=1999; citation_pages=412-417; citation_doi=10.1038/7410; citation_id=CR14
citation_journal_title=Mar Drugs; citation_title=Fucoxanthinol, metabolite of fucoxanthin, improves obesity-induced inflammation in adipocyte cells; citation_author=H Maeda, S Kanno, M Kodate, M Hosokawa, K Miyashita; citation_volume=13; citation_publication_date=2015; citation_pages=4799-4813; citation_doi=10.3390/md13084799; citation_id=CR15
citation_journal_title=Biosci Biotechnol Biochem; citation_title=Characterization of apoptosis induced by fucoxanthin in human promyelocytic leukemia cells; citation_author=E Kotake-Nara, M Terasaki, A Nagao; citation_volume=69; citation_publication_date=2005; citation_pages=224-227; citation_doi=10.1271/bbb.69.224; citation_id=CR16
citation_journal_title=Biochim Biophys Acta; citation_title=Fucoxanthin induces apoptosis and enhances the antiproliferative effect of the PPARgamma ligand, troglitazone, on colon cancer cells; citation_author=M Hosokawa, M Kudo, H Maeda, H Kohno, T Tanaka, K Miyashita; citation_volume=1675; citation_publication_date=2004; citation_pages=113-119; citation_doi=10.1016/j.bbagen.2004.08.012; citation_id=CR17
citation_journal_title=Apoptosis; citation_title=Inhibitors of apoptosis: clinical implications in cancer; citation_author=MS Mohamed, MK Bishr, FM Almutairi, AG Ali; citation_volume=22; citation_publication_date=2017; citation_pages=1487-1509; citation_doi=10.1007/s10495-017-1429-4; citation_id=CR18
citation_journal_title=Nat Rev Mol Cell Biol; citation_title=Molecular mechanisms of caspase regulation during apoptosis; citation_author=SJ Riedl, Y Shi; citation_volume=5; citation_publication_date=2004; citation_pages=897-907; citation_doi=10.1038/nrm1496; citation_id=CR19
Kanazawa K. High bioavailability and diverse biofunctions of Fucoxanthin in Brown algae. Nippon Shokuhin Kagaku Kogaku Kaishi 2012;59:49–55. (in Japanese).
citation_journal_title=Thyroid Res; citation_title=Assessment of Japanese iodine intake based on seaweed consumption in Japan: a literature-based analysis; citation_author=TT Zava, DT Zava; citation_volume=4; citation_publication_date=2011; citation_pages=14; citation_doi=10.1186/1756-6614-4-14; citation_id=CR21
The Ministry of Health, Labour, and Welfare, Japan. In the Ministry of Health, Labour, and Welfare.Daiichi Shuppan Publishing. Tokyo; 2005. Dietary reference intakes for Japanese; pp. 189–193.
citation_journal_title=Mol Pharmacol; citation_title=Epigallocatechin-3-gallate blocks the induction of nitric oxide synthase by down-regulating lipopolysaccharide-induced activity of transcription factor nuclear factor-kappaB; citation_author=YL Lin, JK Lin; citation_volume=52; citation_publication_date=1997; citation_pages=465-472; citation_doi=10.1124/mol.52.3.465; citation_id=CR23
citation_journal_title=Proc Natl Acad Sci U S A; citation_title=Caffeic acid phenethyl ester is a potent and specific inhibitor of activation of nuclear transcription factor NF-kappa B; citation_author=K Natarajan, S Singh, TR Burke, D Grunberger, BB Aggarwal; citation_volume=93; citation_publication_date=1996; citation_pages=9090-9095; citation_doi=10.1073/pnas.93.17.9090; citation_id=CR24
citation_journal_title=Nutr Cancer; citation_title=Anticarcinogenic effects of dried citrus peel in colon carcinogenesis due to inhibition of oxidative stress; citation_author=W Onuma, D Asai, S Tomono, S Miyamoto, G Fujii, T Hamoya, A Nagano, S Takahashi, S Masumori, N Miyoshi, K Wakabayashi, M Mutoh; citation_volume=18; citation_publication_date=2017; citation_pages=1-7; citation_id=CR25