Influence of antigens and adjuvants on the production of gamma‐interferon and antibody by ovine lymphocytes

Immunology and Cell Biology - Tập 68 Số 2 - Trang 127-136 - 1990
D.L. Emery1, J.S. Rothel2, P.R. Wood2
1CSIRO Division of Animal Health, McMaster Laboratory, Private Bag No. 1, Glebe, NSW 2037
2CSIRO Division of Animal Health, Private Bag No. 1, Parkville, Vic. 3052, Australia

Tóm tắt

SummaryThe production of gamma‐interferon (γ‐IFN) and antigen‐specific immunoglobulin isotypes was monitored in sheep given primary and secondary inocula of protein and polysaccharide antigens with or without adjuvants. In efferent lymph from prefemoral nodes draining the site of inoculation, γ‐IFN levels increased within 24 h after injection of adjuvants Quill A, dextran sulfate (DXS) and 6 days after oil adjuvants in saline. The increased synthesis of γ‐IFN was prolonged by 1–2 days by the presence of adjuvanted antigen, but the major stimulus to γ‐IFN production was provided by the adjuvant. Alhydrogel (AH) did not initiate γ‐IFN synthesis. Following a second inocula of antigen in saline, increased levels of γ‐IFN were detected in efferent lymph after 3–4 h, and persisted for at least 2 days. Following cultivation of leucocytes from immunized sheep with specific antigen in vitro, strong positive correlations between levels of interleukin (IL) IL‐2, ‐IFN, proliferative responses and antibody titres were observed. However, after analysis of the antigen‐specific isotypes of immunoglobulin (Ig) there was no correlation between the production of γ‐IFN and particular Ig isotypes. Together with findings that AH did not preferentially induce IgG1 or IgG2, these results suggest that the specific subpopulations of helper T lymphocytes which regulate the production of antibody isotypes by differential secretion of γ‐IFN or IL‐4, are not as clearly defined in sheep as in mice.

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Immunology and Cell Biology

Tập 68 Số 2

127-136

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