Infection of primary cultures of murine splenic and thymic cells with coxsackievirus B4

Microbiology and Immunology - Tập 52 Số 1 - Trang 40-46 - 2008
Hela Jaïdane1,2, Jawhar Gharbi1, Pierre‐Emmanuel Lobert2, Delphine Caloone2, Bernadette Lucas2, Famara Sané2, Thierry Idziorek3, Marie‐Bénédicte Romond4, Mahjoub Aouni1, Didier Hober2
1Laboratory Lab-MDT-01, Unit of Pathogenesis and Viral Virulence, Faculty of Pharmacy of Monastir, Avenue Avicenne 5000 Monastir, Tunisia
2Virology Laboratory/UPRES EA3610, Faculty of Medicine, University of Lille 2, Bâtiment Paul Boulanger, CHRU Lille, 59037 Lille, France
3Inserm U459, Faculty of Medicine
4Laboratory of Bacteriology, Faculty of Pharmaceutical and Biological Sciences, 59037 Lille, France

Tóm tắt

ABSTRACTInfection of primary cultures of total splenic and thymic cells from BALB/c and C3H/HeN mice with CVB4 E2 and JVB strains has been investigated. The presence of positive‐strand viral RNA within cells was determined by semi‐nested RT‐PCR, and viral replication was attested by detection of intracellular negative‐strand viral RNA and by release of infectious particles in culture supernatants. Viral replication occurred with both CVB4 strains to an extent dependent on the genetic background of the host. No interferon‐α production was detected in the supernatants of CVB4‐infected cultures using biological titration. Together these results suggest that infection of splenic and thymic cells can play a role in virus dissemination, and therefore in the pathophysiology of CVB4 infections.

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Microbiology and Immunology

Tập 52 Số 1

40-46

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