Induction of lymphokine‐activated killer and natural killer cell activities from cryopreserved lymphocytes

Transfusion - Tập 28 Số 6 - Trang 531-535 - 1988
Hiroshi Kawai1, Atsushi Komiyama2,1, Miyuki Katoh1,3, Akihiko Yabuhara4,1, Yukiaki Miyagawa1,5, T Akabane1,6
1Department of Pediatrics, Shinshu University School of Medicine, Matsumoto, Japan
2Atsushi Komiyama, MD, PhD, Associate Professor, Department of Pediatrics, Shinshu University School of Medicine.
3Masao Katoh, MD, Postgraduate, Department of Pediatrics, Shinshu University School of Medicine.
4Akihiko Yabuhara, MD, Attending Physician, Department of Pediatrics, Shinshu University School of Medicine.
5Yukiaki Miyagawa, MD, PhD, Resident, Department of Pediatrics, Shinshu University School of Medicine.
6Taro Akabane, MD, PhD, Professor, Department of Pediatrics, Shinshu University School of Medicine.

Tóm tắt

Lymphokine‐activated killer (LAK) and natural killer (NK) cells were studied for their capacity to retain cytotoxicity after cryopreservation. LAK cells were generated by a 4‐day culture of lymphocytes with recombinant interleukin‐2 (rIL‐2). Cytotoxicity was measured by 51Cr‐release assay at effector:target ratios of 10:1 to 80:1. Cryopreserved LAK cells retained 58.8 to 87.4 percent of cytotoxicity, as compared with that in fresh control cells. Cryopreserved NK cell activity against K562 and Molt‐4 targets was 45.7 to 67.9 percent of the respective values of the fresh control cells. The responsiveness of NK cells to polyinosinic‐polycytidilic acid (poly I:C), interferon‐α (IFN‐α), or rIL‐2 remained intact. Activated NK cell activity after poly I:C or IFN‐α stimulation and that after rIL‐2 were, respectively, comparable to and higher than the endogenous NK cell activity of the fresh cells. The composition of lymphocyte subsets as determined by flow cytometry using monoclonal antibodies did not change after cryopreservation, indicating that cellular loss of the given subsets did not occur during the procedure. The retention of substantial levels of cytotoxicity in cryopreserved LAK and NK cells may make them promising candidates as cytotoxic effector cells.

Từ khóa


Tài liệu tham khảo

Sears HF, 1977, Advantages of cryopreserved lymphocytes for sequential evaluation of human immune competence. I. Mitogen stimulation, JNCI, 58, 183, 10.1093/jnci/58.2.183

10.1016/0011-2240(80)90003-6

10.1016/0022-1759(80)90248-3

10.1007/BF00915224

10.1016/0022-1759(85)90041-9

10.1084/jem.155.6.1823

Komiyama A., 1982, Childhood lymphoblastic leukemia with natural killer activity: establishment of the leukemia cell lines retaining the activity, Blood, 60, 1429, 10.1182/blood.V60.6.1429.1429

10.1016/0090-1229(85)90049-2

10.1056/NEJM198512053132327

Papa MZ, 1986, Antitumor efficacy of lymphokine‐activated killer cells and recombinant interleukin‐2 in vivo: Successful immunotherapy of established pulmonary metastases from weakly immunogenic and nonimmunogenic murine tumors of three distinct histological types, Cancer Res, 46, 4973

10.1084/jem.161.1.40

10.1016/0011-2240(77)90129-8

Thông tin
Thông tin xuất bản

Transfusion

Tập 28 Số 6

531-535

Thông tin tác giả