Induction of immediate early genes in the mouse auditory cortex after auditory cued fear conditioning to complex sounds

Genes, Brain and Behavior - Tập 11 Số 3 - Trang 314-324 - 2012
Manuel Peter1, Harald Scheuch1, Thomas R. Burkard1, Jaroslav Tinter1, Tanja Wernle2,1, Simon Rumpel1
1Research Institute of Molecular Pathology, Vienna, Austria
2Present address: Kavli Institute for Systems Neuroscience/Centre for Biology of Memory, Norwegian University of Science and Technology, Trondheim, Norway

Tóm tắt

Immediate early genes (IEGs) are widely used as markers to delineate neuronal circuits because they show fast and transient expression induced by various behavioral paradigms. In this study, we investigated the expression of the IEGs c‐fos and Arc in the auditory cortex of the mouse after auditory cued fear conditioning using quantitative polymerase chain reaction and microarray analysis. To test for the specificity of the IEG induction, we included several control groups that allowed us to test for factors other than associative learning to sounds that could lead to an induction of IEGs. We found that both c‐fos and Arc showed strong and robust induction after auditory fear conditioning. However, we also observed increased expression of both genes in any control paradigm that involved shocks, even when no sounds were presented. Using mRNA microarrays and comparing the effect of the various behavioral paradigms on mRNA expression levels, we did not find genes being selectively upregulated in the auditory fear conditioned group. In summary, our results indicate that the use of IEGs to identify neuronal circuits involved specifically in processing of sound cues in the fear conditioning paradigm can be limited by the effects of the aversive unconditional stimulus and that activity levels in a particular primary sensory cortical area can be strongly influenced by stimuli mediated by other modalities.

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Tài liệu tham khảo

10.1016/0306-4522(93)90369-Q

10.1523/JNEUROSCI.1037-10.2010

10.1523/JNEUROSCI.4737-03.2004

10.1016/S0197-0186(96)00136-2

10.1016/0926-6410(95)90018-7

10.1111/j.1460-9568.2006.04965.x

10.1523/JNEUROSCI.1571-05.2005

10.1007/s00018-009-0109-0

10.1073/pnas.1008604107

10.1016/0306-4522(94)00355-9

10.1016/0304-3940(89)90545-4

10.1038/nrc1209

10.1146/annurev.psych.56.091103.070213

10.1523/JNEUROSCI.23-27-09116.2003

10.1101/lm.183406

10.1016/j.jneumeth.2009.07.015

10.1038/16046

10.1523/JNEUROSCI.21-14-05089.2001

10.1046/j.0953-816x.2001.01531.x

10.1126/science.1164139

10.1101/lm.86104

10.1016/j.neuron.2009.01.015

10.1037/a0012902

10.1016/j.nlm.2008.06.006

10.1016/j.neuropharm.2006.07.028

10.1007/978-1-4615-0263-0

10.1016/j.neuron.2010.06.015

10.1038/nature10674

10.1038/nature09736

10.1073/pnas.92.12.5734

10.1006/meth.2001.1262

10.1016/0896-6273(95)90299-6

10.1523/JNEUROSCI.4639-08.2009

10.1016/j.modgep.2007.06.006

10.1146/annurev.neuro.24.1.897

10.1098/rspb.1970.0040

10.1016/S0006-8993(97)01266-3

10.1002/(SICI)1097-4695(19990205)38:2<234::AID-NEU6>3.0.CO;2-G

10.1126/science.3037702

10.1016/S0896-6273(00)80375-X

Paxinos G.&Franklin K.B.J.(2001)The mouse brain in stereotaxic coordinates 2nd edn. Academic Press.

10.1523/JNEUROSCI.18-18-07452.1998

10.1126/science.1143839

Romanski L.M., 1992, Equipotentiality of thalamo‐amygdala and thalamo‐cortico‐amygdala circuits in auditory fear conditioning., J Neurosci, 12, 4501, 10.1523/JNEUROSCI.12-11-04501.1992

10.1016/S0168-0102(97)00095-3

10.1523/JNEUROSCI.11-08-02321.1991

Smyth G.K., 2004, Linear models and empirical bayes methods for assessing differential expression in microarray experiments., Stat Appl Genet Mol Biol, 3, 10.2202/1544-6115.1027

Song E.Y., 2010, Auditory cortex is important in the extinction of two different tone‐based conditioned fear memories in rats., Front Behav Neurosci, 4, 24.

10.1007/s003590050140

10.1186/1471-2105-7-106

10.2144/000112776

10.1016/j.cell.2006.06.038

10.1038/nature01262

10.1038/nrn1366

10.1016/S0169-328X(02)00556-9

10.1186/ar2338

10.1186/1756-6606-4-4

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Genes, Brain and Behavior

Tập 11 Số 3

314-324

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